p21 Recombinant monoclonal antibody

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  WB analysis using 85435-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85435-2-RR (Cdkn1a antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of NIH/3T3 using 85435-2-RR

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using Cdkn1a antibody (85435-2-RR, Clone: 242478H2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity of 85435-2-RR

  Biolayer interferometry (BLl) kinetic assays of 85435-2-RR against Mouse p21 were performed. The affinity constant is below 1 pM.

p21 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 85435-2-RR

Clone No.242478H2

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IF/ICC, ELISA

Synonyms

Cdkn1a, Cdkn1, P21 protein, p21Cip1, p21WAF

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, Neuro-2a cells, DC2.4 cells, RAW 264.7 cells
• Positive IF/ICC detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85435-2-RR targets p21 in WB, IF/ICC, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag28249

  Product name: Recombinant mouse Cdkn1a protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-159 aa of NM_001111099

  Sequence: MSNPGDVRPVPHRSKVCRCLFGPVDSEQLRRDCDALMAGCLQEARERWNFDFVTETPLEGNFVWERVRSLGLPKVYLSPGSRSRDDLGGDKRPSTSSALLQGPAPEDHVALSLSCTLVSERPEDSPGGPGTSQGRKRRQTSLTDFYHSKRRLVFCKRKP

• Full Name: cyclin-dependent kinase inhibitor 1A (P21)
• Calculated Molecular Weight: 18 kDa
• Observed Molecular Weight: 15-18 kDa
• GenBank Accession Number: NM_001111099
• Gene Symbol: p21
• Gene ID (NCBI): 12575
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P39689
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDKN1A (p21, CIP1, WAF1) is a cyclin-dependent kinase inhibitor. CDKN1A binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at the G1 phase. The expression of CDKN1A is induced by wild-type but not mutant p53 protein, through which CDKN1A mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. CDKN1A can interact with proliferating cell nuclear antigen (PCNA), and plays a regulatory role in S phase DNA replication and DNA damage repair. CDKN1A was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation.