p70(S6K) Polyclonal antibody

p70(S6K) Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 26587-1-AP

Synonyms

p70 alpha, p70 ribosomal S6 kinase alpha, p70 S6 kinase alpha, p70 S6K, p70 S6K 1, p70 S6K alpha, p70 S6KA, p70(S6K), p70(S6K) alpha, p70S6k, P70S6K1, PS6K, Ribosomal protein S6 kinase I, RPS6KB1, S6K, S6K beta 1, S6K1, STK14A



Tested Applications

Positive WB detected inMCF-7 cells, A549 cells
Positive IP detected inA549 cells
Positive IHC detected inhuman lung cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 26587-1-AP is p70(S6K) Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen p70(S6K) fusion protein Ag24073
Full Name ribosomal protein S6 kinase, 70kDa, polypeptide 1
Calculated molecular weight 59 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC053365
Gene symbol RPS6KB1
Gene ID (NCBI) 6198
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RPS6KB1(Ribosomal protein S6 kinase beta-1) is also named as STK14A, p70 S6KA and belongs to the S6 kinase subfamily. RPS6KB1 is a major substrate of MTOR and acts as a crucial effector of MTOR signaling pathway. It plays a key role in cell growth and proliferation by regulating INS sensitivity, metabolism, protein synthesis, and cell cycle. RPS6KB1 may play an important role in the progression of HCC and could serve as a potential molecular target for HCC therapy (PMID:22684641). RPS6KB1 is a 70 kDa protein and has 5 isoforms with the calculated molecular mass of 51-59kDa produced by alternative initiation.

Protocols

Product Specific Protocols
WB protocol for p70(S6K) antibody 26587-1-APDownload protocol
IHC protocol for p70(S6K) antibody 26587-1-APDownload protocol
IF protocol for p70(S6K) antibody 26587-1-APDownload protocol
IP protocol for p70(S6K) antibody 26587-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
pigWB

Am J Physiol Endocrinol Metab

Prematurity blunts the feeding-induced stimulation of translation initiation signaling and protein synthesis in muscle of neonatal piglets.

Authors - Jane K Naberhuis