pan-keratin Polyclonal antibody

pan-keratin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

Publications

39

Cat no : 26411-1-AP

Synonyms

pan-CK, CK 5, Cytokeratin 5, CK-5, keratin 5

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Validation Data Gallery

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  WB analysis using 26411-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26411-1-AP (pan-keratin antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

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  IHC staining of human oesophagus using 26411-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 26411-1-AP (pan-keratin antibody) at dilution of 1:3000 (under 10x lens)..

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  IHC staining of human oesophagus using 26411-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 26411-1-AP (pan-keratin antibody) at dilution of 1:3000 (under 40x lens)..

• 

  IHC staining of human brown disease using 26411-1-AP

  Immunohistochemical analysis of paraffin-embedded human brown disease slide using 26411-1-AP (pan-keratin antibody) at dilution of 1:20000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brown disease using 26411-1-AP

  Immunohistochemical analysis of paraffin-embedded human brown disease slide using 26411-1-AP (pan-keratin antibody) at dilution of 1:20000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human renal cell carcinoma using 26411-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 26411-1-AP (pan-keratin antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human renal cell carcinoma using 26411-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 26411-1-AP (pan-keratin antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 26411-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 26411-1-AP (pan-keratin antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 26411-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 26411-1-AP (pan-keratin antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 26411-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26411-1-AP (pan-keratin antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 26411-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26411-1-AP (pan-keratin antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 26411-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26411-1-AP (pan-keratin antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 26411-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26411-1-AP (pan-keratin antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 26411-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26411-1-AP (pan-keratin antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 26411-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26411-1-AP (pan-keratin antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 26411-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue slide using 26411-1-AP (pan-keratin antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 26411-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue slide using 26411-1-AP (pan-keratin antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 26411-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 26411-1-AP (pan-keratin antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 26411-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 26411-1-AP (pan-keratin antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 26411-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26411-1-AP (pan-keratin antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 26411-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26411-1-AP (pan-keratin antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human colon cancer using 26411-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using pan-keratin antibody (26411-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CD8 antibody (66868-1-Ig, Clone: 1G2B10, green).

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  IF Staining of human colon cancer using 26411-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using pan-keratin antibody (26411-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CD8 antibody (66868-1-Ig, Clone: 1G2B10, green).

• 

  IF Staining of HeLa using 26411-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using pan-keratin antibody (26411-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 26411-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using pan-keratin antibody (26411-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of A431 using 26411-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using pan-keratin antibody (26411-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A431 cells, MCF-7 cells, HeLa cells
• Positive IHC detected in: human oesophagus tissue, human brown disease, human cervical cancer tissue, human liver tissue, human lung cancer tissue, human renal cell carcinoma tissue, human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human colon cancer tissue
• Positive IF/ICC detected in: A431 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:50000-1:200000
• Immunohistochemistry (IHC): IHC : 1:1500-1:6000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 14 publications below
• IF: See 22 publications below

Product Information

26411-1-AP targets pan-keratin in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat, sheep
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: pan-keratin fusion protein Ag24184
• Full Name: keratin 5
• Calculated Molecular Weight: 590 aa, 62 kDa
• Observed Molecular Weight: 46-58 kDa
• GenBank Accession Number: BC024292
• Gene Symbol: Cytokeratin 5
• Gene ID (NCBI): 3852
• RRID: AB_2880505
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Pan-keratin, also known as pan-cytokeratin, refers to a group of antibodies that recognize a broad array of keratin proteins. Keratins are a large family of intermediate filament (IF) proteins that provide structural integrity to epithelial cells. These proteins are essential for maintaining cell shape, strength, and resilience . The keratin gene family is divided into two major groups: type I keratins (acidic) and type II keratins (basic), with 27 type I and 26 type II keratin genes in humans. Pan-keratin immunostaining is widely used in pathology and research due to its ability to detect a variety of keratin proteins, making it a valuable tool for identifying epithelial cells and tissues. This staining technique is particularly useful in the diagnosis of tumors, as it can help distinguish between epithelial and non-epithelial neoplasms. This antibody is a pan-keratin antibody.

Publications

Species	Application	Title
human	IF	Nat Commun; The estrogen response in fibroblasts promotes ovarian metastases of gastric cancer; Authors - Simeng Hu
human	IF	Biomaterials; Heterotypic 3D pancreatic cancer model with tunable proportion of fibrotic elements.; Authors - Hiroyoshi Y Tanaka
mouse	IF	Biomaterials; MSC-derived exosomes protect against oxidative stress-induced skin injury via adaptive regulation of the NRF2 defense system.; Authors - Tian Wang
	IHC	J Nanobiotechnology; ZNPs reduce epidermal mechanical strain resistance by promoting desmosomal cadherin endocytosis via mTORC1-TFEB-BLOC1S3 axis; Authors - Xuan Lai
human	IF	Oncogene; The keratin 17/YAP/IL6 axis contributes to E-cadherin loss and aggressiveness of diffuse gastric cancer; Authors - Mengjie Li
mouse	IHC	Oncogene; USP13 promotes development and metastasis of high-grade serous ovarian carcinoma in a novel mouse model.; Authors - Juntae Kwon