Phospho-TDP43 (Ser403/404) Monoclonal antibody

Phospho-TDP43 (Ser403/404) Monoclonal Antibody for WB, FC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse and More (1)

Applications

WB, IF, FC, IHC, ELISA

Conjugate

Unconjugated

Publications

9

CloneNo.

6B11B12

Reviews (1)

Cat no : 66079-1-Ig

Synonyms

ALS10, Phospho-TDP43 (Ser403/404), TAR DNA binding protein, TAR DNA binding protein 43, TARDBP, TDP 43, TDP43, tdp-43

---

Validation Data Gallery

• 

  WB analysis using 66079-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66079-1-Ig (Phospho-TDP43 (Ser403/404) antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis of K-562 using 66079-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 66079-1-Ig (Phospho-TDP43 (Ser403/404) Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 66079-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66079-1-Ig (Phospho-TDP43 (Ser403/404) Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 66079-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66079-1-Ig (Phospho-TDP43 (Ser403/404) Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66079-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66079-1-Ig (Phospho-TDP43 (Ser403/404) Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of Jurkat using 66079-1-Ig

  1X10^6 Jurkat cells were stained with 0.20ug Phospho-TDP43 (Ser403/404) antibody (66079-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH.

---

Tested Applications

• Positive WB detected in: HEK-293 cells, Jurkat cells, K-562 cells, RAW 264.7 cells, fetal human brain tissue
• Positive FC detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC): FC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 3 publications below
• IF: See 3 publications below

Product Information

66079-1-Ig targets Phospho-TDP43 (Ser403/404) in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, zebrafish
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: TAR DNA binding protein
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: NM_007375
• Gene Symbol: TARDBP
• Gene ID (NCBI): 23435
• RRID: AB_11183767
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Transactivation response (TAR) DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43) was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (PMID: 17023659,19823856, 21666678, 22193176).66079-1-Ig is a mouse monoclonal antibody recognizing TDP-43 only when phosphorylated at 403/404. Immunohistochemical analyses using this antibody only stain the insoluble inclusions in pathologic tissues without normal diffuse nuclear staining.

Publications

Species	Application	Title
human,zebrafish	IHC,IF	Nat Neurosci; TDP-43 condensates and lipid droplets regulate the reactivity of microglia and regeneration after traumatic brain injury; Authors - Alessandro Zambusi
human	IHC	Acta Neuropathol Commun; Influence of APOE genotype in primary age-related tauopathy.; Authors - Andrew C Robinson
human	WB	Eur J Med Chem; Targeting nuclear protein TDP-43 by cell division cycle kinase 7 inhibitors: A new therapeutic approach for amyotrophic lateral sclerosis.; Authors - Elisa Rojas-Prats
human	IF	Biochemistry; Using Tetracysteine-Tagged TDP-43 with a Biarsenical Dye To Monitor Real-Time Trafficking in a Cell Model of Amyotrophic Lateral Sclerosis.; Authors - Janice S W Ng
human	WB	PLoS One; Drosophila lines with mutant and wild type human TDP-43 replacing the endogenous gene reveals phosphorylation and ubiquitination in mutant lines in the absence of viability or lifespan defects.; Authors - Jer-Cherng Chang
human	WB	Methods Mol Biol; Sedimentation Assays to Assess the Impact of Posttranslational Modifications on Phase Separation of RNA-Binding Proteins In Vitro and In Cells; Authors - Lara A Gruijs da Silva

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Azita (Verified Customer) (06-17-2021)

Immunocytochemistry labelling of (4% PFA) fixed NSC-34 cells by Phospho-TDP43 (Ser403/404) Monoclonal antibody at dilution of 1:100 showed strong labelling.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: NSC-34 cells