Tested Applications
| Positive WB detected in | HEK-293 cells, Jurkat cells, K-562 cells, RAW 264.7 cells, fetal human brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66079-1-Ig targets Phospho-TDP43 (Ser403/404) in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, pig, monkey, zebrafish |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | TAR DNA binding protein |
| Calculated Molecular Weight | 43 kDa |
| Observed Molecular Weight | 25 kDa |
| GenBank Accession Number | NM_007375 |
| Gene Symbol | TDP-43 |
| Gene ID (NCBI) | 23435 |
| RRID | AB_11183767 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q13148 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Transactivation response (TAR) DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43) was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (PMID: 17023659,19823856, 21666678, 22193176).66079-1-Ig is a mouse monoclonal antibody recognizing TDP-43 only when phosphorylated at 403/404. Immunohistochemical analyses using this antibody only stain the insoluble inclusions in pathologic tissues without normal diffuse nuclear staining.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-TDP43 (Ser403/404) antibody 66079-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-TDP-43 antibody (Cat# 66079-1-Ig) has 13 citations spanning journals including Cell, Cell Stem Cell, Nature Neuroscience, EMBO Journal, Acta Neuropathologica Communications, European Journal of Medicinal Chemistry, Scientific Reports, PLoS ONE, Biochemistry, Biochemical and Biophysical Research Communications, Methods in Molecular Biology, and Research Square. Research fields encompass amyotrophic lateral sclerosis (ALS), frontotemporal dementia, TDP-43 phosphorylation and phase separation, traumatic brain injury, neurodegeneration, and radiation-induced neuropathology across human, mouse, zebrafish, pig, and non-human primate models.
| Species | Application | Title |
|---|---|---|
Cell Stem Cell KCNJ2 inhibition mitigates mechanical injury in a human brain organoid model of traumatic brain injury | ||
Nat Neurosci TDP-43 condensates and lipid droplets regulate the reactivity of microglia and regeneration after traumatic brain injury | ||
EMBO J Disease-linked TDP-43 hyperphosphorylation suppresses TDP-43 condensation and aggregation. | ||
Eur J Med Chem Targeting nuclear protein TDP-43 by cell division cycle kinase 7 inhibitors: A new therapeutic approach for amyotrophic lateral sclerosis. | ||
Acta Neuropathol Commun CK1δ/ε-mediated TDP-43 phosphorylation contributes to early motor neuron disease toxicity in amyotrophic lateral sclerosis |
Reviews
What customers say
The single review reports a 5-star rating for this Phospho-TDP43 (Ser403/404) monoclonal antibody. Using a 1:100 dilution on 4% PFA-fixed NSC-34 cells, the reviewer observed strong labeling in immunocytochemistry. Overall, the feedback is positive, indicating reliable performance and clear signal at a low dilution.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Azita (Verified Customer) (06-17-2021) | Immunocytochemistry labelling of (4% PFA) fixed NSC-34 cells by Phospho-TDP43 (Ser403/404) Monoclonal antibody at dilution of 1:100 showed strong labelling.
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