• HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human IL-15 protein- GMP grade

EC50

0.07-0.37 ng/mL EC50

Specific Activity

4.4x10^7 IU/mg calibrated against IL-15 reference standard (NIBSC cat no: 95/554). Reference use only. Specific activity is not routinely tested

Species Reactivity

human

Purity

>95%

ISO 13485 certified HumanKine® human cell-expressed cytokines and growth factors

Cat no : HZ-1323-GMP

Synonyms

Interleukin 15, IL15, IL-15MGC9721, Interleukin-15



Technical Specifications

GeneID 3600
Species Human
Expression HEK293
EC50 0.07-0.37 ng/mL EC50
Specific Activity4.4x10^7 IU/mg calibrated against IL-15 reference standard (NIBSC cat no: 95/554). Reference use only. Specific activity is not routinely tested
Purity >95%
Endotoxin< 0.1 EU/µg
Accession NumberP40933
Molecular Mass 11-23 kDa
Formulation 50 mM Acetate pH 4.0 + 150mM NaCl
Species Reactivity human

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1x PBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

GMP Quality Policies

HumanKine® GMP (Good Manufacturing Practice) recombinant proteins are manufactured and validated in accordance with ISO 13485 quality management system and is compliant with GMP.
Our GMP recombinant proteins are animal component free (ACF), xeno free (XF) and tag free (TF). Read more about these policies here.

Background

IL-15 is a 4-α-helix bundle cytokine playing a pivotal role in stimulation of both innate and adaptive immune cells. It is produced primarily by keratinocytes, skeletal muscle cells, monocytes, and CD4+ T cells. It is a member of the common gamma chain family. The members of the common gamma chain family include the IL-2, IL-4, IL-7, IL-9, and IL-21 and require binding to the common  chain receptor for activation. It can be used in growth and maintenance of T and NK cells. It is also shown to be used in proliferation and functional effect of T cells for adoptive cell therapy. It is a glycosylated protein and HumanKine IL-15 appear as 12.5-25 kDa bands (PMID: 24587813, 26627006, 27849617, 31250350).

Synonyms

Interleukin 15, IL15, IL-15MGC9721, Interleukin-15