Validation Data Gallery
Technical Specifications
| GeneID | 3624 |
| Species | Human |
| Expression | HEK293 |
| Activity(Primary) | 0.5-3.5 ng/mL |
| Specific Activity | minimally 4.00 x 102 IU/mg |
| Purity | >95% |
| Endotoxin | <1 EU/μg |
| Accession Number | P08476 |
| Molecular Mass | 13kDa reduced, 22 kDa non-reduced, homodimer, non-glycosylated |
| Formulation | 1x PBS, See Certificate of Analysis for details |
| Species Reactivity | human,mouse |
Stability and Reconstitution
| Stability and Storage | Product Form | Temperature Conditions | Storage Time (From Date of Receipt) |
|---|---|---|---|
| Lyophilized | -20°C to -80°C | Until Expiry Date | |
| Lyophilized | Room Temperature | 2 weeks | |
| Reconstituted as per CofA | -20°C to -80°C | 6 months | |
| Reconstituted as per CofA | 4°C | 1 week | |
| Avoid repeated freeze-thaw cycles. | |||
| Reconstitution | Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently Swirl or tap vial to mix |
Background
HumanKine Activin A is a high-quality, animal-free recombinant human protein widely used in stem cell research, cell culture, and signaling pathway studies. Produced in HEK293 cells, it is designed to deliver exceptional purity, strong bioactivity, and low endotoxin levels for consistent experimental performance. HumanKine Activin A supports applications such as stem cell differentiation, tissue development, regenerative medicine research, and advanced biological assay development. Activin A is a dimeric growth factor within the TGF-β superfamily that engages specific type II and type I receptors to initiate SMAD2/3 signaling cascades and downstream transcriptional regulation. Functionally, Activin A is a key regulator of pluripotent stem cell maintenance and a potent inducer of definitive endoderm formation Its reliable human-like glycosylation profile makes it a preferred choice for researchers seeking reproducible results in sensitive cell-based workflows.(PMID 16885530, 8200957)
Synonyms
Activin A, Activin beta A chain, EDF, FRP, INHBA, Inhibin beta A chain, inhibin, beta A
Publications
| Species | Title |
|---|---|
Nat Commun Selectively targeting the AdipoR2-CaM-CaMKII-NOS3 axis by SCM-198 as a rapid-acting therapy for advanced acute liver failure | |
Cell Res Visualization of aging-associated chromatin alterations with an engineered TALE system. | |
Biomater Res Self-organized insulin-producing β-cells differentiated from human omentum-derived stem cells and their in vivo therapeutic potential | |
EMBO J HIF1α induced switch from bivalent to exclusively glycolytic metabolism during ESC-to-EpiSC/hESC transition. | |
Nat Protoc The generation of kidney organoids by differentiation of human pluripotent cells to ureteric bud progenitor-like cells. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH ay (Verified Customer) (12-11-2025) | HumanKine Activin A worked very well for producing 3D tubular structures from iPS cells in my setup. It reconstitutes without any trouble, and the solution has held up at 4°C for close to two weeks. I still plan to check vascular marker expression, but so far the results look very promising.
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FH toy (Verified Customer) (12-11-2025) | I successfully used the HumanKine Activin A to generate 3D tubular structures from iPS cells with excellent results. The lyophilized powder resuspends easily and remained stable at 4°C for nearly two weeks. I still need to confirm vascular marker expression, but the early outcomes are very promising.
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FH MO (Verified Customer) (12-11-2025) | Verified users specifically cite effective differentiation of iPS and embryonic stem cells, indicating reliable performance for cell culture applications
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FH Ryszard (Verified Customer) (03-21-2025) | I was able to use this humankine Activin A to great succes to generate 3D tubular structures derived from iPS cells. The powder is easy to resuspend and seems stable at 4C for almost 2 weeks. I will check for Vascular markers at somepoint but looks very promising.
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FH Enes Yagiz (Verified Customer) (08-23-2022) | It is able differentiate human embryonic stemcells into primitive endoderm
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FH Tobias (Verified Customer) (09-13-2021) | HumanKine Activin A is a great product for stem cells, allowing successful differentiation.
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