HumanKine® recombinant human BMP-4 protein- GMP grade

• HEK293 expressed
• Endotoxin-free
• Animal-component free

EC50

1.5-9 ng/mL

Species Reactivity

human,mouse

Purity

>95%

Reviews (3)

Cat no : HZ-1045-GMP

Synonyms

BMP 2B, BMP 4, BMP2B, BMP2B1, BMP4, BMP-4, Bone morphogenetic protein 2B, bone morphogenetic protein 4, DVR4, MCOPS6, ZYME

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Validation Data Gallery

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  Purity of recombinant human BMP-4 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

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  Recombinant human BMP-4 (HZ-1045-GMP) stimulates dose-dependent induction of alkaline phosphatase production in the ATDC-5 mouse chondrogenic cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. ATDC-5 cells were treated with increasing concentrations of recombinant human BMP-4 for 72 hrs hours before lysis and addition of pNPP. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 values range from 1.5-9 ng/ml.

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Technical Specifications

• GeneID: 652
• Species: Human
• Expression: HEK293
• EC50: 1.5-9 ng/mL
• Purity: >95%
• Endotoxin: <0.1 EU/µg
• Accession Number: P12644
• Molecular Mass: 20 to 24 kDa reduced, 32 to 38 kDa non-reduced, homodimer, glycosylated
• Formulation: 2x PBS + 6% Ethanol, See Certificate of Analysis for details
• Species Reactivity: human,mouse

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

• Reconstitution: Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 4 mM HCl containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

GMP Quality Policies

HumanKine® GMP (Good Manufacturing Practice) recombinant proteins are manufactured and validated in accordance with ISO 13485 quality management system and is compliant with GMP.
Our GMP recombinant proteins are animal component free (ACF), xeno free (XF) and tag free (TF). Read more about these policies here.

Whitepapers

Humankine BMP4 has authentic protein processing and high activity

Background

Bone morphogenetic protein-4 (BMP-4), also known as BMP2B and DVR4, belongs to the TGF-beta family. BMPs have been shown to promote astroglial differentiation both in vitro and in vivo. BMP-4 can also induce cartilage and bone formation (PMID 16234975). Xeno-free Recombinant Human BMP-4 is expressed in human 293 cells as a disulfide linked homodimeric glycoprotein with an apparent molecular mass of 34 kDa.

Synonyms

BMP 2B, BMP 4, BMP2B, BMP2B1, BMP4, BMP-4, Bone morphogenetic protein 2B, bone morphogenetic protein 4, DVR4, MCOPS6, ZYME

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Jose (Verified Customer) (02-09-2024)

Shipping was fast and product as of yet is working well and as needed, You all should look into getting your COAs on the site, and maybe searchable so that if the paper copy is lost you can look up COA by lot number and it will generate the PDF so as not to have to wait for a response from you all.

• Applications: Cell culture

FH

Xunyu (Verified Customer) (07-26-2022)

Satisfied

• Applications: Cell culture

FH

Xunyu (Verified Customer) (06-13-2022)

satisfied

• Applications: Cell culture