Live Chat
You've been automatically redirected here from Humanzyme.com, which is now part of Proteintech Group. Click here to dismiss this alert.
  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

Animal-free Recombinant Human BMP-4

Activity

Typically ≤ 10 ng/mL EC50

Species Reactivity

Human

Purity

>95%

Cat no : HZ-1045

Synonyms

BMP-2B, DVR4 BMP-4, BMP4, BMP 4

Product data gallery View All

  • BMP-4 activity assay

    The activity was determined by the dose-dependent induction of alkaline phosphatase production in the ATDC-5 cell line (Mouse chondrogenic cell line) using pNPP as chromogenic substrate.

  • BMP-4 activity assay

    The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue. R represents reducing conditions and NR represents non-reducing conditions.

Technical Specifications

GeneID 652
Species Human
Expression HEK293
Activity Typically ≤ 10 ng/mL EC50
Purity >95%
Molecular Mass 34 kDa, homodimer, glycosylated
Formulation 2x PBS + 6% Ethanol, See Certificate of Analysis for details
Species Reactivity human,  mouse

Stability and Reconstitution

Stability and Storage Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20oC) and (-80oC). Upon reconstitution we recommend that the solution can be stored at (4oC) for short term or at (-20oC) to (-80oC) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 4 mM HCl containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Product Description

Animal-free Recombinant Human BMP-4 is expressed in human 293 cells as a disulfide linked homodimeric glycoprotein with an apparent molecular mass of 34 kDa. This cytokine is produced in a serum-free, chemically defined media.

Background

Bone morphogenetic protein-4 (BMP4), also known as BMP2B and DVR4, belongs to the TGF-beta family. BMPs have been shown to promote astroglial differentiation both in vitro and in vivo. BMP4 can also induce cartilage and bone formation (PMID 16234975). Xeno-free Recombinant Human BMP-4 is expressed in human 293 cells as a disulfide linked homodimeric glycoprotein with an apparent molecular mass of 34 kDa. 

Synonyms

BMP-2B, DVR4 BMP-4, BMP4, BMP 4

Publications

SpeciesTitle
human

Stem Cell Res Ther

Clonal derivation of white and brown adipocyte progenitor cell lines from human pluripotent stem cells.

Authors - Michael D West
View Article
human

J Biol Chem

Transforming growth factor β (TGF-β)-induced up-regulation of TGF-β receptors at the cell surface amplifies the TGF-β response.

Authors - Dana Duan
View Article
human

Biotechnol Bioeng

Optimized protocol for the hepatic differentiation of induced pluripotent stem cells in a fluidic microenvironment.

Authors - Mathieu Danoy
View Article
human

J Tissue Eng Regen Med

Transplantation of multi-ciliated airway cells derived from human iPS cells using an artificial tracheal patch into rat trachea.

Authors - Hideaki Okuyama
View Article

Mol Omics

Analysis of the transcription factors and their regulatory roles during a step-by-step differentiation of induced pluripotent stem cells into hepatocyte-like cells.

Authors - Yannick Tauran
View Article

Tissue Eng Part A

Enhanced hepatic differentiation of human iPS cells using gas permeable membrane.

Authors - Keiichi Kimura
View Article