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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human BMP-4 protein

Activity

1.5-9 ng/mL EC50

Species Reactivity

human

Purity

>95%

Cat no : HZ-1045

Synonyms

BMP 2B, BMP 4, BMP2B, BMP2B1, BMP4, BMP-4, Bone morphogenetic protein 2B, bone morphogenetic protein 4, DVR4, MCOPS6, ZYME

Validation Data Gallery

  • The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

    The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

  • Recombinant human BMP-4 (HZ-1045) stimulates dose-dependent induction of alkaline phosphatase production in the ATDC-5 mouse chondrogenic cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. ATDC-5 cells were treated with increasing concentrations of recombinant human BMP-4 for 72 hrs hours before lysis and addition of pNPP. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 values range from 1.5-9 ng/ml.

    Recombinant human BMP-4 (HZ-1045) stimulates dose-dependent induction of alkaline phosphatase production in the ATDC-5 mouse chondrogenic cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. ATDC-5 cells were treated with increasing concentrations of recombinant human BMP-4 for 72 hrs hours before lysis and addition of pNPP. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 values range from 1.5-9 ng/ml.

Technical Specifications

GeneID 652
Species human
Expression HEK293
Activity 1.5-9 ng/mL EC50
Purity >95%
Endotoxin<1 EU/µg
Accession NumberP12644
Molecular Mass 34 kDa, homodimer, glycosylated
Formulation 2x PBS + 6% Ethanol, See Certificate of Analysis for details
Species Reactivity human,mouse

Stability and Reconstitution

Stability and Storage Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 4 mM HCl containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Background

Bone morphogenetic protein-4 (BMP4), also known as BMP2B and DVR4, belongs to the TGF-beta family. BMPs have been shown to promote astroglial differentiation both in vitro and in vivo. BMP4 can also induce cartilage and bone formation (PMID 16234975). Xeno-free Recombinant Human BMP-4 is expressed in human 293 cells as a disulfide linked homodimeric glycoprotein with an apparent molecular mass of 34 kDa. 

Synonyms

BMP 2B, BMP 4, BMP2B, BMP2B1, BMP4, BMP-4, Bone morphogenetic protein 2B, bone morphogenetic protein 4, DVR4, MCOPS6, ZYME

Publications

SpeciesTitle
human

Stem Cell Res Ther

Clonal derivation of white and brown adipocyte progenitor cell lines from human pluripotent stem cells.

Authors - Michael D West
View Article
human

J Biol Chem

Transforming growth factor β (TGF-β)-induced up-regulation of TGF-β receptors at the cell surface amplifies the TGF-β response.

Authors - Dana Duan
View Article
human

Biotechnol Bioeng

Optimized protocol for the hepatic differentiation of induced pluripotent stem cells in a fluidic microenvironment.

Authors - Mathieu Danoy
View Article
human

J Tissue Eng Regen Med

Transplantation of multi-ciliated airway cells derived from human iPS cells using an artificial tracheal patch into rat trachea.

Authors - Hideaki Okuyama
View Article

Mol Omics

Analysis of the transcription factors and their regulatory roles during a step-by-step differentiation of induced pluripotent stem cells into hepatocyte-like cells.

Authors - Yannick Tauran
View Article

Tissue Eng Part A

Enhanced hepatic differentiation of human iPS cells using gas permeable membrane.

Authors - Keiichi Kimura
View Article