HumanKine® recombinant human BMP-4 protein

• HEK293 expressed
• Endotoxin-free
• Animal-component free

Activity(Primary)

1.5-9 ng/mL

Species Reactivity

human,mouse

Purity

>95%

Publications

81

Reviews (1)

Cat no : HZ-1045

Synonyms

BMP 2B, BMP 4, BMP2B, BMP2B1, BMP4, BMP-4, Bone morphogenetic protein 2B, bone morphogenetic protein 4, DVR4, MCOPS6, ZYME

---

Validation Data Gallery

• 

  Purity of recombinant human BMP-4 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

• 

  Recombinant human BMP-4 (HZ-1045) stimulates dose-dependent induction of alkaline phosphatase production in the ATDC-5 mouse chondrogenic cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. ATDC-5 cells were treated with increasing concentrations of recombinant human BMP-4 for 72 hours before lysis and addition of pNPP. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 values range from 1.5-9 ng/ml.

• 

  Proteintech BMP-4 (HZ-1045) demonstrates equivalent induction and 2-fold lower EC50 compared to leading competitors. Recombinant human BMP-4 stimulates dose-dependent induction of alkaline phosphatase production in the ATDC-5 mouse chondrogenic cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. ATDC-5 cells were treated with increasing concentrations of recombinant human BMP-4 for 72 hours before lysis and addition of pNPP. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 values range from 1.5-9 ng/ml.

• 

  Human iPSC-derived microglia (white) residing within an in vivo brain-like organoid environment (blue) derived using HumanKine growth factors (TGF beta 1- HZ-1011, BMP4- HZ-1045, and Thrombopoietin HZ-1248).(Credits- Credit: Simon T. Schafer & Monique Pena, Technical University of Munich, Center for Organoid Systems)

• 

  Human induced pluripotent cells(iPSCs) were differentiated to definitive endoderm using HumanKine growth factors (Activin A- HZ-1138, bFGF-HZ-1285, BMP-4- HZ-1045, and Wnt3A-HZ-1296). The differentiation was confirmed by detection of expression of FOXA2(Red) and downregulation of pluripotency marker NANOG(yellow), in immunofluorescence

---

Technical Specifications

• GeneID: 652
• Species: Human
• Expression: HEK293
• Activity(Primary): 1.5-9 ng/mL
• Purity: >95%
• Endotoxin: <1 EU/µg
• Accession Number: P12644
• Molecular Mass: 20 to 24 kDa reduced, 32 to 38 kDa non-reduced, homodimer, glycosylated
• Formulation: 2x PBS + 6% Ethanol, See Certificate of Analysis for details
• Species Reactivity: human,mouse

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

• Reconstitution: Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 4 mM HCl containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Whitepapers

Humankine BMP4 has authentic protein processing and high activity

Background

Bone morphogenetic protein-4 (BMP4), also known as BMP2B and DVR4, belongs to the TGF-beta family. BMPs have been shown to promote astroglial differentiation both in vitro and in vivo. BMP4 can also induce cartilage and bone formation (PMID 16234975). Xeno-free Recombinant Human BMP-4 is expressed in human 293 cells as a disulfide linked homodimeric glycoprotein with an apparent molecular mass of 34 kDa. 

Synonyms

BMP 2B, BMP 4, BMP2B, BMP2B1, BMP4, BMP-4, Bone morphogenetic protein 2B, bone morphogenetic protein 4, DVR4, MCOPS6, ZYME

Publications

Species	Title
mouse	Cell; Generation of Blastocyst-like Structures from Mouse Embryonic and Adult Cell Cultures.; Authors - Ronghui Li
human	Nat Methods; Long-term expansion of alveolar stem cells derived from human iPS cells in organoids.; Authors - YukiYamamoto
human	Gastroenterology; The RNA-binding protein RBPMS2 regulates development of gastrointestinal smooth muscle.; Authors - CécileNotarnicola
human	Nat Commun; Selectively targeting the AdipoR2-CaM-CaMKII-NOS3 axis by SCM-198 as a rapid-acting therapy for advanced acute liver failure; Authors - Rui Wang
human	Cell Res; Visualization of aging-associated chromatin alterations with an engineered TALE system.; Authors - Ruotong Ren
human	Blood; Expression of BMPRIA on human thymic NK cell precursors: role of BMP signaling in intrathymic NK cell development.; Authors - LauraHidalgo

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Angeles (Verified Customer) (02-09-2023)

It works fine

• Applications: Cell culture
• Cell Tissue Type: Lymphocytes, Dendritic cells, Macrophages