HumanKine® recombinant human BMP-4 protein

• HEK293 expressed
• Endotoxin-free
• Animal-component free

Activity

1.5-9 ng/mL EC50

Species Reactivity

human,mouse

Purity

>95%

Publications

57

Cat no : HZ-1045

Synonyms

BMP 2B, BMP 4, BMP2B, BMP2B1, BMP4, BMP-4, Bone morphogenetic protein 2B, bone morphogenetic protein 4, DVR4, MCOPS6, ZYME

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Validation Data Gallery

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  The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

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  Recombinant human BMP-4 (HZ-1045) stimulates dose-dependent induction of alkaline phosphatase production in the ATDC-5 mouse chondrogenic cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. ATDC-5 cells were treated with increasing concentrations of recombinant human BMP-4 for 72 hrs hours before lysis and addition of pNPP. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 values range from 1.5-9 ng/ml.

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Technical Specifications

• GeneID: 652
• Species: Human
• Expression: HEK293
• Activity: 1.5-9 ng/mL EC50
• Purity: >95%
• Endotoxin: <1 EU/µg
• Accession Number: P12644
• Molecular Mass: 34 kDa, homodimer, glycosylated
• Formulation: 2x PBS + 6% Ethanol, See Certificate of Analysis for details
• Species Reactivity: human,mouse

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 4 mM HCl containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Whitepapers

Humankine BMP4 has authentic protein processing and high activity

Background

Bone morphogenetic protein-4 (BMP4), also known as BMP2B and DVR4, belongs to the TGF-beta family. BMPs have been shown to promote astroglial differentiation both in vitro and in vivo. BMP4 can also induce cartilage and bone formation (PMID 16234975). Xeno-free Recombinant Human BMP-4 is expressed in human 293 cells as a disulfide linked homodimeric glycoprotein with an apparent molecular mass of 34 kDa. 

Synonyms

BMP 2B, BMP 4, BMP2B, BMP2B1, BMP4, BMP-4, Bone morphogenetic protein 2B, bone morphogenetic protein 4, DVR4, MCOPS6, ZYME

Publications

Species	Title
human	Stem Cell Res Ther; Clonal derivation of white and brown adipocyte progenitor cell lines from human pluripotent stem cells.; Authors - Michael D West
human	J Biol Chem; Transforming growth factor β (TGF-β)-induced up-regulation of TGF-β receptors at the cell surface amplifies the TGF-β response.; Authors - Dana Duan
human	Biotechnol Bioeng; Optimized protocol for the hepatic differentiation of induced pluripotent stem cells in a fluidic microenvironment.; Authors - Mathieu Danoy
human	J Tissue Eng Regen Med; Transplantation of multi-ciliated airway cells derived from human iPS cells using an artificial tracheal patch into rat trachea.; Authors - Hideaki Okuyama
	Mol Omics; Analysis of the transcription factors and their regulatory roles during a step-by-step differentiation of induced pluripotent stem cells into hepatocyte-like cells.; Authors - Yannick Tauran
	Tissue Eng Part A; Enhanced hepatic differentiation of human iPS cells using gas permeable membrane.; Authors - Keiichi Kimura