Validation Data Gallery
Technical Specifications
| GeneID | 2252 |
| Species | Human |
| Expression | HEK293 |
| Activity(Primary) | 4-20 ng/mL |
| Specific Activity | minimally 2.00 x 105 IU/mg |
| Purity | >95% |
| Endotoxin | <1 EU/μg |
| Accession Number | P21781 |
| Molecular Mass | 17 and 25 to 30 kDa reduced, 17 and 24 to 29 kDa non-reduced, monomer, glycosylated |
| Formulation | 1x PBS, See Certificate of Analysis for details |
| Species Reactivity | human,monkey |
Stability and Reconstitution
| Stability and Storage | Product Form | Temperature Conditions | Storage Time (From Date of Receipt) |
|---|---|---|---|
| Lyophilized | -20°C to -80°C | Until Expiry Date | |
| Lyophilized | Room Temperature | 2 weeks | |
| Reconstituted as per CofA | -20°C to -80°C | 6 months | |
| Reconstituted as per CofA | 4°C | 1 week | |
| Avoid repeated freeze-thaw cycles. | |||
| Reconstitution | Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix. |
Background
Fibroblast Growth Factor 7 (FGF-7), also known as Keratinocyte Growth Factor (KGF), is a potent epithelial cell-specific growth factor that binds selectively to receptors expressed on epithelial cells. It plays a critical role in tissue repair, epithelial cell proliferation, and differentiation, making it highly relevant for regenerative medicine and advanced cell culture systems. FGF-7 is widely used in epithelial stem cell maintenance, as well as in the development of bladder and lung organoids. In addition, it is a key growth factor in protocols for the differentiation of pluripotent stem cells into pancreatic endocrine progenitor cells, a promising approach for treating Type 1 Diabetes. Due to its epithelial specificity and biological activity, FGF-7 is an essential component in organoid culture, stem cell differentiation, and cell therapy workflows. HumanKine FGF-7 is expressed in HEK293 human cells, ensuring authentic human post-translational modifications (PTMs) that enhance protein folding, stability, and bioactivity compared to non-mammalian expression systems.
Synonyms
FGF 7, FGF7, FGF-7, Fibroblast growth factor 7, HBGF 7, Keratinocyte growth factor, KGF
Publications
| Species | Title |
|---|---|
J Invest Dermatol Increased KGF expression promotes fibroblast activation in a double paracrine manner resulting in cutaneous fibrosis. | |
Stem Cell Res Ther Bronchioalveolar stem cells derived from mouse-induced pluripotent stem cells promote airway epithelium regeneration. | |
Biomed Pharmacother Regulation of keratinocyte proliferation and differentiation by secoiridoid oleacein in monoculture and fibroblast co-culture models | |
Exp Cell Res Directed differentiation of human embryonic stem cells into conjunctival epithelial cells | |
Phytomedicine Kaempferol inhibits SARS-CoV-2 invasion by impairing heptad repeats-mediated viral fusion | |
Redox Biol PNKP targeting engages the autophagic machinery through STING and STAT3 to potentiate ferroptosis and chemotherapy in TNBC |