HumanKine® recombinant human IL-1 beta protein

• HEK293 expressed
• Endotoxin-free
• Animal-component free

Activity

Typically ≤ 0.05 ng/mL EC50

Species Reactivity

human,mouse

Purity

>95%

Publications

24

Cat no : HZ-1164

Synonyms

Catabolin, IL 1, IL 1 beta, IL1 BETA, IL-1 beta, IL1B, IL-1b, IL1beta, IL1F2, Interleukin 1 beta, interleukin 1, beta

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Validation Data Gallery

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  The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

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  The activity was determined by the dose-dependent stimulation of the proliferation of mouse D10S cells using Promega CellTiter96® Aqueous Non-Radioactive Cell Proliferation Assay.

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Technical Specifications

• GeneID: 3553
• Species: Human
• Expression: HEK293
• Activity: Typically ≤ 0.05 ng/mL EC50
• Purity: >95%
• Endotoxin: <1 EU/µg
• Accession Number: P01584
• Molecular Mass: 18 and 25 kDa, monomer, glycosylated
• Formulation: 1x PBS, See Certificate of Analysis for details
• Species Reactivity: human,mouse

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Whitepapers

Effective Differentiation of Th17 Cells With HumanKine® Cytokines Expressed in Human Cells

Time-Saving Dendritic Cell Generation No Medium Change - Less Reagents With HumanKine® GM-CSF and HumanKine IL4

Background

Interleukin-1 is a pro-inflammatory cytokine with multiple biological effects. The IL-1 gene family encodes three proteins: IL-1α, IL-1β and their naturally occurring inhibitor Il-1RN. Interleukin 1β(IL-1β), mainly produced by blood monocytes and tissue macrophages, has been implicated in mediating both acute and chronic inflammation. IL-1β is known to be involved in a variety of cellular activities, including cell proliferation, differentiation and apoptosis. IL-1β is emerging as a key mediator of carcinogenesis that characterizes host-environment interactions (PMID: 8630372; PMID: 12401481; PMID: 23704929; PMID: 24618930).

Synonyms

Catabolin, IL 1, IL 1 beta, IL1 BETA, IL-1 beta, IL1B, IL-1b, IL1beta, IL1F2, Interleukin 1 beta, interleukin 1, beta

Publications

Species	Title
human	Mediators Inflamm; Matrix Metalloproteinase-9-Dependent Release of IL-1β by Human Eosinophils.; Authors - Stephane Esnault
human	Biosci Trends; Analysis of cytotoxicity induced by proinflammatory cytokines in the human alveolar epithelial cell line A549.; Authors - MitsuakiMuroya
human	BMC Musculoskelet Disord; Effect of interleukin-1β treatment on co-cultures of human meniscus cells and bone marrow mesenchymal stromal cells.; Authors - AnikaChowdhury
human	PLoS One; Mesenchymal stromal cell-derived extracellular vesicles reduce lung inflammation and damage in nonclinical acute lung injury: Implications for COVID-19.; Authors - Caryn Cloer
human	Life Sci; Cynaropicrin is dual regulator for both degradation factors and synthesis factors in the cartilage metabolism.; Authors - TeruakiMasutani
human	Int Immunopharmacol; Autonomous cure of damaged human intestinal epithelial cells by TLR2 and TLR4-dependent production of IL-22 in response to Spirulina polysaccharides.; Authors - AkiraTominaga