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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human IL-1 beta protein

Activity

Typically ≤ 0.05 ng/mL EC50

Species Reactivity

human

Purity

>95%

Cat no : HZ-1164

Synonyms

Catabolin, IL 1, IL 1 beta, IL1 BETA, IL-1 beta, IL1B, IL-1b, IL1beta, IL1F2, Interleukin 1 beta, interleukin 1, beta

Validation Data Gallery

  • The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

    The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

  • The activity was determined by the dose-dependent stimulation of the proliferation of mouse D10S cells using Promega CellTiter96® Aqueous Non-Radioactive Cell Proliferation Assay.

    The activity was determined by the dose-dependent stimulation of the proliferation of mouse D10S cells using Promega CellTiter96® Aqueous Non-Radioactive Cell Proliferation Assay.

Technical Specifications

GeneID 3553
Species human
Expression HEK293
Activity Typically ≤ 0.05 ng/mL EC50
Purity >95%
Molecular Mass 18 and 25 kDa, monomer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human, mouse

Stability and Reconstitution

Stability and Storage Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Background

Interleukin-1 is a pro-inflammatory cytokine with multiple biological effects. The IL-1 gene family encodes three proteins: IL-1α, IL-1β and their naturally occurring inhibitor Il-1RN. Interleukin 1β(IL-1β), mainly produced by blood monocytes and tissue macrophages, has been implicated in mediating both acute and chronic inflammation. IL-1β is known to be involved in a variety of cellular activities, including cell proliferation, differentiation and apoptosis. IL-1β is emerging as a key mediator of carcinogenesis that characterizes host-environment interactions (PMID: 8630372; PMID: 12401481; PMID: 23704929; PMID: 24618930).

Synonyms

Catabolin, IL 1, IL 1 beta, IL1 BETA, IL-1 beta, IL1B, IL-1b, IL1beta, IL1F2, Interleukin 1 beta, interleukin 1, beta

Publications

SpeciesTitle
human

Mediators Inflamm

Matrix Metalloproteinase-9-Dependent Release of IL-1β by Human Eosinophils.

Authors - Stephane Esnault
View Article
human

Biosci Trends

Analysis of cytotoxicity induced by proinflammatory cytokines in the human alveolar epithelial cell line A549.

Authors - MitsuakiMuroya
View Article
human

BMC Musculoskelet Disord

Effect of interleukin-1β treatment on co-cultures of human meniscus cells and bone marrow mesenchymal stromal cells.

Authors - AnikaChowdhury
View Article
human

Life Sci

Cynaropicrin is dual regulator for both degradation factors and synthesis factors in the cartilage metabolism.

Authors - TeruakiMasutani
View Article
human

Int Immunopharmacol

Autonomous cure of damaged human intestinal epithelial cells by TLR2 and TLR4-dependent production of IL-22 in response to Spirulina polysaccharides.

Authors - AkiraTominaga
View Article
human

J Intensive Care

Synergistic cytoprotection by co-treatment with dexamethasone and rapamycin against proinflammatory cytokine-induced alveolar epithelial cell injury.

Authors - Ken Kuwajima
View Article