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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

Animal-free Recombinant Human IL-1 beta

Activity

Typically ≤ 0.05 ng/mL EC50

Species Reactivity

human

Purity

>95%

Cat no : HZ-1164

Synonyms

Catabolin, IL 1, IL 1 beta, IL1 BETA, IL-1 beta, IL1B, IL-1b, IL1beta, IL1F2, Interleukin 1 beta, interleukin 1, beta



Technical Specifications

GeneID 3553
Species human
Expression HEK293
Activity Typically ≤ 0.05 ng/mL EC50
Purity >95%
Molecular Mass 18 and 25 kDa, monomer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human, mouse

Stability and Reconstitution

Stability and Storage Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20oC) and (-80oC). Upon reconstitution we recommend that the solution can be stored at (4oC) for short term or at (-20oC) to (-80oC) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Product Description

Animal-free Recombinant Human IL-1 beta expressed in human 293 cells has a very high activity with a typical EC50 of ≤0.1 ng/mL using a bioassay to measure stimulation of the proliferation of mouse D10S cells. In comparison with the E. coli expressed protein, IL-1 beta is 50% more potent promoting human CD4+ T cell differentiation into Th17 cells. This cytokine is important for inflammatory responses. It is also involved in a variety of cellular activities which includes cell differentiation, proliferation, and apoptosis. It is produced in a serum-free, chemically defined media.

Background

Interleukin-1 is a pro-inflammatory cytokine with multiple biological effects. The IL-1 gene family encodes three proteins: IL-1α, IL-1β and their naturally occurring inhibitor Il-1RN. Interleukin 1β(IL-1β), mainly produced by blood monocytes and tissue macrophages, has been implicated in mediating both acute and chronic inflammation. IL-1β is known to be involved in a variety of cellular activities, including cell proliferation, differentiation and apoptosis. IL-1β is emerging as a key mediator of carcinogenesis that characterizes host-environment interactions (PMID: 8630372; PMID: 12401481; PMID: 23704929; PMID: 24618930).

Synonyms

Catabolin, IL 1, IL 1 beta, IL1 BETA, IL-1 beta, IL1B, IL-1b, IL1beta, IL1F2, Interleukin 1 beta, interleukin 1, beta

Publications

SpeciesTitle
human

Mediators Inflamm

Matrix Metalloproteinase-9-Dependent Release of IL-1β by Human Eosinophils.

Authors - Stephane Esnault
human

Biosci Trends

Analysis of cytotoxicity induced by proinflammatory cytokines in the human alveolar epithelial cell line A549.

Authors - MitsuakiMuroya
human

BMC Musculoskelet Disord

Effect of interleukin-1β treatment on co-cultures of human meniscus cells and bone marrow mesenchymal stromal cells.

Authors - AnikaChowdhury
human

Life Sci

Cynaropicrin is dual regulator for both degradation factors and synthesis factors in the cartilage metabolism.

Authors - TeruakiMasutani
human

Int Immunopharmacol

Autonomous cure of damaged human intestinal epithelial cells by TLR2 and TLR4-dependent production of IL-22 in response to Spirulina polysaccharides.

Authors - AkiraTominaga
human

PLoS One

Characterization of protective human CD4CD25 FOXP3 regulatory T cells generated with IL-2, TGF-β and retinoic acid.

Authors - LingLu