- HEK293 expressed
- Endotoxin-free
- Animal-component free
Animal-free Recombinant Human IL-1 beta
Cat no : HZ-1164
Synonyms
Catabolin, Lymphocyte-activating factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF) Lymphocyte activating factor (LAF)
Validation Data Gallery View All
Technical Specifications
| GeneID | 3553 |
| Species | Human |
| Expression | HEK293 |
| Activity | Typically ≤ 0.05 ng/mL EC50 |
| Purity | >95% |
| Molecular Mass | 18 and 25 kDa, monomer, glycosylated |
| Formulation | 1x PBS, See Certificate of Analysis for details |
| Species Reactivity | human, mouse |
Stability and Reconstitution
| Stability and Storage | Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20oC) and (-80oC). Upon reconstitution we recommend that the solution can be stored at (4oC) for short term or at (-20oC) to (-80oC) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products. |
| Reconstitution | Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA). |
Product Description
Animal-free Recombinant Human IL-1 beta expressed in human 293 cells has a very high activity with a typical EC50 of ≤0.1 ng/mL using a bioassay to measure stimulation of the proliferation of mouse D10S cells. In comparison with the E. coli expressed protein, IL-1 beta is 50% more potent promoting human CD4+ T cell differentiation into Th17 cells. This cytokine is important for inflammatory responses. It is also involved in a variety of cellular activities which includes cell differentiation, proliferation, and apoptosis. It is produced in a serum-free, chemically defined media.
Background
Interleukin-1 is a pro-inflammatory cytokine with multiple biological effects. The IL-1 gene family encodes three proteins: IL-1α, IL-1β and their naturally occurring inhibitor Il-1RN. Interleukin 1β(IL-1β), mainly produced by blood monocytes and tissue macrophages, has been implicated in mediating both acute and chronic inflammation. IL-1β is known to be involved in a variety of cellular activities, including cell proliferation, differentiation and apoptosis. IL-1β is emerging as a key mediator of carcinogenesis that characterizes host-environment interactions (PMID: 8630372; PMID: 12401481; PMID: 23704929; PMID: 24618930).
Synonyms
Catabolin, Lymphocyte-activating factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF) Lymphocyte activating factor (LAF)
Publications
| Species | Title |
|---|---|
Mediators Inflamm Matrix Metalloproteinase-9-Dependent Release of IL-1β by Human Eosinophils. | |
Biosci Trends Analysis of cytotoxicity induced by proinflammatory cytokines in the human alveolar epithelial cell line A549. | |
BMC Musculoskelet Disord Effect of interleukin-1β treatment on co-cultures of human meniscus cells and bone marrow mesenchymal stromal cells. | |
Life Sci Cynaropicrin is dual regulator for both degradation factors and synthesis factors in the cartilage metabolism. | |
Int Immunopharmacol Autonomous cure of damaged human intestinal epithelial cells by TLR2 and TLR4-dependent production of IL-22 in response to Spirulina polysaccharides. | |
PLoS One Characterization of protective human CD4CD25 FOXP3 regulatory T cells generated with IL-2, TGF-β and retinoic acid. |
