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Animal-free Recombinant Human M-CSF
Humankine®5 Publications

Catalog number: HZ-1192

HEK293 expressed Endotoxin-free Animal-component free

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Freight/Packing £00.00

Format: Lyophilized

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Technical Specifications

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M-CSF activity assay

The activity was determined by the dose-dependent stimulation of the proliferation of murine M-NFS-60 cells (Mouse Myeloid Leukemia indicator cell line) using Promega CellTiter96® Aqueous Non-Radioactive Cell Proliferation Assay.

M-CSF purity

The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue. R represents reducing conditions and NR represents non-reducing conditions.

GeneID:
1435
Species:
Human
Expression:
HEK293
Activity:
Typically ≤ 4 ng/mL EC50
Purity:
>95%
Molecular Mass:
35 and 40 kDa, homodimer, glycosylated
Formulation
1x PBS, See Certificate of Analysis for details
Species Reactivity:
human,  mouse

Stability and Reconstitution

Stability and Storage:
Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20oC) and (-80oC). Upon reconstitution we recommend that the solution can be stored at (4oC) for short term or at (-20oC) to (-80oC) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.


Reconstitution:
Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Product Description

Product Description:

Animal-free Recombinant Human M-CSF is expressed in human 293 cells and has an apparent molecular mass of 35 to 40 kDa due to glycosylation. M-CSF is a potent hematopoietic factor produced by a variety of cells including lymphocytes, monocytes, fibroblasts, endothelial cells, myoblasts, and osteoblasts. It is a key regulator of cellular proliferation, differentiation, and survivial of blood monocytes, tissue macrophages, and their progenitor cells. This cytokine is produced in a human cell expression system with serum-free, chemically defined media.



Background:

M-CSF plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes. M-CSF promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. It also plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. On the cellular level, M-CSF promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration and plays a role in lipoprotein clearance (PMID: 29734839; 29679908; 28202039)



Synonyms:
Macrophage Colony Stimulating Factor, CSF-1, MGI-IM MCSF, M CSF, CSF1, CSF 1, MGIIM, MGI IM



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