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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human M-CSF protein

Activity

Typically ≤ 4 ng/mL EC50

Species Reactivity

human

Purity

>95%

Cat no : HZ-1192

Synonyms

CSF 1, CSF1, Lanimostim, M CSF, MCSF, M-CSF

Validation Data Gallery

  • The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

    The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

  • The activity was determined by the dose-dependent stimulation of the proliferation of murine M-NFS-60 cells (Mouse Myeloid Leukemia indicator cell line) using Promega CellTiter96® Aqueous Non-Radioactive Cell Proliferation Assay.

    The activity was determined by the dose-dependent stimulation of the proliferation of murine M-NFS-60 cells (Mouse Myeloid Leukemia indicator cell line) using Promega CellTiter96® Aqueous Non-Radioactive Cell Proliferation Assay.

Technical Specifications

GeneID 1435
Species human
Expression HEK293
Activity Typically ≤ 4 ng/mL EC50
Purity >95%
Endotoxin<1 EU/µg
Molecular Mass 35 and 40 kDa, homodimer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human,  mouse

Stability and Reconstitution

Stability and Storage Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Background

M-CSF plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes. M-CSF promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. It also plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. On the cellular level, M-CSF promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration and plays a role in lipoprotein clearance (PMID: 29734839; 29679908; 28202039)

Synonyms

CSF 1, CSF1, Lanimostim, M CSF, MCSF, M-CSF

Publications

SpeciesTitle
mouse

Pharm Biol

β-Sitosterol modulates macrophage polarization and attenuates rheumatoid inflammation in mice.

Authors - Rui Liu
View Article
mouse

Acta Biomater

Exposure to hydroxyapatite nanoparticles enhances Toll-like receptor 4 signal transduction and overcomes endotoxin tolerance in vitro and in vivo.

Authors - Yuchen Hua
View Article
human

J Biotechnol

Comparison of osteoclastogenesis and resorption activity of human osteoclasts on tissue culture polystyrene and on natural extracellular bone matrix in 2D and 3D.

Authors - CKleinhans
View Article
mouse

Arthritis Res Ther

Differential regulation of osteoclastogenesis by Notch2/Delta-like 1 and Notch1/Jagged1 axes.

Authors - ChiyokoSekine
View Article
mouse

Biochim Biophys Acta Mol Basis Dis

Extracellular HMGB1 augments macrophage inflammation by facilitating the endosomal accumulation of ALD-DNA via TLR2/4-mediated endocytosis.

Authors - Jincheng Lu
View Article
mouse

Cell Death Dis

Gpr174-deficient regulatory T cells decrease cytokine storm in septic mice.

Authors - Dongze Qiu
View Article