HumanKine® recombinant human M-CSF protein

• HEK293 expressed
• Endotoxin-free
• Animal-component free

Activity

0.7-4.0 ng/mL EC50

Species Reactivity

human,mouse

Purity

>95%

Publications

10

Cat no : HZ-1192

Synonyms

CSF 1, CSF1, Lanimostim, M CSF, MCSF, M-CSF

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Validation Data Gallery

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  Purity of recombinant human M-CSF was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

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  Recombinant human M-CSF (Cat no: HZ-1192) stimulates dose-dependent proliferation of the murine mouse myloid leukemia (M-NFS-60) cell line. Cell number was quantitatively assessed by Prestoblue® Cell Viability Reagent. M-NFS-60 cells were treated with increasing concentrations of recombinant M-CSF for 48 hours. The EC50 range is 0.7-4.0 ng/mL.

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Technical Specifications

• GeneID: 1435
• Species: Human
• Expression: HEK293
• Activity: 0.7-4.0 ng/mL EC50
• Purity: >95%
• Endotoxin: <1 EU/µg
• Accession Number: P09603
• Molecular Mass: 35 and 40 kDa, homodimer, glycosylated
• Formulation: 1x PBS, See Certificate of Analysis for details
• Species Reactivity: human,mouse

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Background

M-CSF plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes. M-CSF promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. It also plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. On the cellular level, M-CSF promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration and plays a role in lipoprotein clearance (PMID: 29734839; 29679908; 28202039)

Synonyms

CSF 1, CSF1, Lanimostim, M CSF, MCSF, M-CSF

Publications

Species	Title
mouse	Pharm Biol; β-Sitosterol modulates macrophage polarization and attenuates rheumatoid inflammation in mice.; Authors - Rui Liu
mouse	Acta Biomater; Exposure to hydroxyapatite nanoparticles enhances Toll-like receptor 4 signal transduction and overcomes endotoxin tolerance in vitro and in vivo.; Authors - Yuchen Hua
human	J Biotechnol; Comparison of osteoclastogenesis and resorption activity of human osteoclasts on tissue culture polystyrene and on natural extracellular bone matrix in 2D and 3D.; Authors - CKleinhans
mouse	Int Immunopharmacol; Berberine suppresses cecal ligation and puncture induced intestinal injury by enhancing Treg cell function.; Authors - Dongze Qiu
mouse	Arthritis Res Ther; Differential regulation of osteoclastogenesis by Notch2/Delta-like 1 and Notch1/Jagged1 axes.; Authors - ChiyokoSekine
mouse	Biochim Biophys Acta Mol Basis Dis; Extracellular HMGB1 augments macrophage inflammation by facilitating the endosomal accumulation of ALD-DNA via TLR2/4-mediated endocytosis.; Authors - Jincheng Lu