- HEK293 expressed
- Endotoxin-free
- Animal-component free
HumanKine® recombinant human M-CSF protein
Cat no : HZ-1192
Synonyms
CSF 1, CSF1, Lanimostim, M CSF, MCSF, M-CSF
Validation Data Gallery
Technical Specifications
GeneID | 1435 |
Species | Human |
Expression | HEK293 |
Activity | 0.7-4.0 ng/mL EC50 |
Purity | >95% |
Endotoxin | <1 EU/µg |
Accession Number | P09603 |
Molecular Mass | 35 and 40 kDa, homodimer, glycosylated |
Formulation | 1x PBS, See Certificate of Analysis for details |
Species Reactivity | human,mouse |
Stability and Reconstitution
Stability and Storage | Product Form | Temperature Conditions | Storage Time (From Date of Receipt) |
---|---|---|---|
Lyophilized | -20°C to -80°C | Until Expiry Date | |
Lyophilized | Room Temperature | 2 weeks | |
Reconstituted as per CofA | -20°C to -80°C | 6 months | |
Reconstituted as per CofA | 4°C | 1 week | |
Avoid repeated freeze-thaw cycles. |
Reconstitution | Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA). |
Background
M-CSF plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes. M-CSF promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. It also plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. On the cellular level, M-CSF promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration and plays a role in lipoprotein clearance (PMID: 29734839; 29679908; 28202039)
Synonyms
CSF 1, CSF1, Lanimostim, M CSF, MCSF, M-CSF
Publications
Species | Title |
---|---|
Pharm Biol β-Sitosterol modulates macrophage polarization and attenuates rheumatoid inflammation in mice. | |
Acta Biomater Exposure to hydroxyapatite nanoparticles enhances Toll-like receptor 4 signal transduction and overcomes endotoxin tolerance in vitro and in vivo. | |
J Biotechnol Comparison of osteoclastogenesis and resorption activity of human osteoclasts on tissue culture polystyrene and on natural extracellular bone matrix in 2D and 3D. | |
Int Immunopharmacol Berberine suppresses cecal ligation and puncture induced intestinal injury by enhancing Treg cell function. | |
Arthritis Res Ther Differential regulation of osteoclastogenesis by Notch2/Delta-like 1 and Notch1/Jagged1 axes. | |
Biochim Biophys Acta Mol Basis Dis Extracellular HMGB1 augments macrophage inflammation by facilitating the endosomal accumulation of ALD-DNA via TLR2/4-mediated endocytosis. |