Animal-free Recombinant Human M-CSF is expressed in human 293 cells and has an apparent molecular mass of 35 to 40 kDa due to glycosylation. M-CSF is a potent hematopoietic factor produced by a variety of cells including lymphocytes, monocytes, fibroblasts, endothelial cells, myoblasts, and osteoblasts. It is a key regulator of cellular proliferation, differentiation, and survivial of blood monocytes, tissue macrophages, and their progenitor cells. This cytokine is produced in a human cell expression system with serum-free, chemically defined media.

M-CSF plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes. M-CSF promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. It also plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. On the cellular level, M-CSF promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration and plays a role in lipoprotein clearance (PMID: 29734839; 29679908; 28202039)