• HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human M-CSF protein

Activity

0.7-4.0 ng/mL EC50

Species Reactivity

human,mouse

Purity

>95%

Cat no : HZ-1192

Synonyms

CSF 1, CSF1, Lanimostim, M CSF, MCSF, M-CSF



Technical Specifications

GeneID 1435
Species Human
Expression HEK293
Activity 0.7-4.0 ng/mL EC50
Purity >95%
Endotoxin<1 EU/µg
Accession NumberP09603
Molecular Mass 35 and 40 kDa, homodimer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human,mouse

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Background

M-CSF plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes. M-CSF promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. It also plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. On the cellular level, M-CSF promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration and plays a role in lipoprotein clearance (PMID: 29734839; 29679908; 28202039)

Synonyms

CSF 1, CSF1, Lanimostim, M CSF, MCSF, M-CSF

Publications

SpeciesTitle
mouse

Cell Metab

Senescent immune cells release grancalcin to promote skeletal aging.

Authors - Chang-Jun Li
mouse

Cell Metab

A mechanosensitive lipolytic factor in the bone marrow promotes osteogenesis and lymphopoiesis.

Authors - Hui Peng
human

Autophagy

Induction of a Na+/K+-ATPase-dependent form of autophagy triggers preferential cell death of human immunodeficiency virus type-1-infected macrophages.

Authors - Gang Zhang
human

Arthritis Rheum

The pattern-recognition receptor nucleotide-binding oligomerization domain--containing protein 1 promotes production of inflammatory mediators in rheumatoid arthritis synovial fibroblasts.

Authors - KazuhiroYokota
mouse

Cell Death Dis

Gpr174-deficient regulatory T cells decrease cytokine storm in septic mice.

Authors - Dongze Qiu
mouse

Biochim Biophys Acta Mol Basis Dis

Extracellular HMGB1 augments macrophage inflammation by facilitating the endosomal accumulation of ALD-DNA via TLR2/4-mediated endocytosis.

Authors - Jincheng Lu