• HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human M-CSF protein- GMP grade

EC50

0.7-4.0 ng/mL EC50

Specific Activity

1.2x10^8 IU/mg calibrated against M-CSF reference standard (NIBSC cat no: 89/512). Reference use only. Specific activity is not routinely tested

Species Reactivity

human,mouse

Purity

>95%

ISO 13485 certified HumanKine® human cell-expressed cytokines and growth factors

Cat no : HZ-1192-GMP

Synonyms

CSF 1, CSF1, Lanimostim, M CSF, MCSF, M-CSF



Technical Specifications

GeneID 1435
Species Human
Expression HEK293
EC50 0.7-4.0 ng/mL EC50
Specific Activity1.2x10^8 IU/mg calibrated against M-CSF reference standard (NIBSC cat no: 89/512). Reference use only. Specific activity is not routinely tested
Purity >95%
Endotoxin<0.1 EU/µg
Accession NumberP09603
Molecular Mass 35 and 40 kDa, homodimer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human,mouse

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

GMP Quality Policies

HumanKine® GMP (Good Manufacturing Practice) recombinant proteins are manufactured and validated in accordance with ISO 13485 quality management system and is compliant with GMP.
Our GMP recombinant proteins are animal component free (ACF), xeno free (XF) and tag free (TF). Read more about these policies here.

Background

M-CSF plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes. M-CSF promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. It also plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. On the cellular level, M-CSF promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration and plays a role in lipoprotein clearance (PMID: 29734839; 29679908; 28202039)

Synonyms

CSF 1, CSF1, Lanimostim, M CSF, MCSF, M-CSF