Validation Data Gallery
Technical Specifications
| GeneID | 6469 |
| Species | Human |
| Expression | HEK293 |
| Activity(Primary) | Typically ≤350 ng/mL |
| Purity | >90% |
| Endotoxin | <1.0 EU/μg |
| Accession Number | Q15465 |
| Molecular Mass | 19-22 kDa reduced and non-reduced, monomer, glycosylated |
| Formulation | 10mM Tris pH 7.4 + 100mM NaCl + 1% CHAPS, See Certificate of Analysis for details |
| Species Reactivity | human,mouse |
Stability and Reconstitution
| Stability and Storage | Product Form | Temperature Conditions | Storage Time (From Date of Receipt) |
|---|---|---|---|
| Lyophilized | -20°C to -80°C | Until Expiry Date | |
| Lyophilized | Room Temperature | 2 weeks | |
| Reconstituted as per CofA | -20°C to -80°C | 6 months | |
| Reconstituted as per CofA | 4°C | 1 week | |
| Avoid repeated freeze-thaw cycles. | |||
| Reconstitution | Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.1 mg/mL in sterile water containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix. |
Background
SHH, also named as HHG-1, belongs to the hedgehog family. SHH binds to the patched receptor (PTC), that in association with smoothened (SMO) activate the transcription of target genes. HumanKine SHH is a high-quality, animal-free recombinant Sonic Hedgehog protein designed for advanced stem cell and neurodevelopment research. In brain organoid differentiation, SHH plays a key morphogenic role by helping guide ventral patterning, supporting the generation of midbrain and ventral neural lineages, and enabling more physiologically relevant brain model development. HumanKine SHH expressed in HEK293 cells offers key advantages including native human conformation, high bioactivity, low endotoxin, xeno-free production, and strong lot-to-lot consistency. These features make it a reliable choice for researchers seeking reproducible results in organoid workflows, neural differentiation, and developmental signaling studies.
Synonyms
HHG 1, HHG1, HLP3, HPE3, MCOPCB5, SHH, SMMCI, Sonic hedgehog protein, TPT, TPTPS
Publications
| Species | Title |
|---|---|
Mol Biol Rep Discovery of YS-1 as a cell line of gastric inflammatory cancer-associated fibroblasts | |
EBioMedicine Leukemia inhibitory factor is a therapeutic target for renal interstitial fibrosis | |
Int J Mol Sci FAM20B-Catalyzed Glycosylation Regulates the Chondrogenic and Osteogenic Differentiation of the Embryonic Condyle by Controlling IHH Diffusion and Release |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Alessandro (Verified Customer) (07-27-2022) | Essential supplement for mzOPCs differentiation
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FH Adeeb (Verified Customer) (12-06-2021) | I used the sonic hedgehog to upregulate HCC using HepG2 cells, as a positive control when testing the hypothesis that Nrf2 also stimulates increased tumour burden. The product worked well and is something that we would consider using again.
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FH Toby (Verified Customer) (07-28-2021) | We are investigating HCC tumour burden. The sonic hedgehog pathway has been shown to be significantly involved in HCC. We have used this humankine recombinant protein as a means to induce/boost the sonic hedgehog pathway within both HCC and CRC cell lines - we have then measured the proliferative rate of these cell lines to identify its effect on cellular proliferation. It has shown promising results thus far.
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