Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CCR6 antibody (66801-1-Ig, Clone: 1C11A9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human CCR6 (66801-1-Ig, Clone:1C11A9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66801-1-Ig (CCR6 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66801-1-Ig (CCR6 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CCR6 antibody (66801-1-Ig, Clone: 1C11A9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CCR6 antibody (66801-1-Ig, Clone: 1C11A9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66801-1-PBS in a different storage buffer formulation.

1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human CCR6 (66801-1-Ig, Clone:1C11A9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66801-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66801-1-Ig (CCR6 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66801-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66801-1-Ig (CCR6 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66801-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CCR6 antibody (66801-1-Ig, Clone: 1C11A9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66801-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 CCR6 antibody (CL594-66801, Clone: 1C11A9 ) at dilution of 1:200.

Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 CCR6 antibody (CL594-66801, Clone: 1C11A9 ) at dilution of 1:200.

HeLa cells and A431 cells were subjected to SDS PAGE followed by western blot with 66611-1-Ig (CCRL2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66611-1-Ig (CCRL2 antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66611-1-Ig (CCRL2 antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

A431 cells were subjected to SDS PAGE followed by western blot with 13387-1-AP (CCRL2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

1X10^6 A431 cells were stained with .2ug CCRL2 antibody (13387-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

CCR6 fusion protein Ag24185 SDS-PAGE

CCR6 fusion protein Ag24195 SDS-PAGE

HeLa cells and A431 cells were subjected to SDS PAGE followed by western blot with 66611-1-Ig (CCRL2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66611-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66611-1-Ig (CCRL2 antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66611-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66611-1-Ig (CCRL2 antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66611-1-PBS in a different storage buffer formulation.