CCR6 Monoclonal antibody

CCR6 Monoclonal Antibody for IF, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

IHC, IF

Conjugate

Unconjugated

CloneNo.

1C11A9

Cat no : 66801-1-Ig

Synonyms

BN 1, C C chemokine receptor type 6, C C CKR 6, CC CKR 6, CCR 6, CCR6, CD196, Chemokine receptor like 3, CKR L3, CKR6, CKRL3, CMKBR6, DCR2, DRY 6, DRY6, G protein coupled receptor 29, GPR CY4, GPR29, GPRCY4, LARC receptor, STRL22

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Validation Data Gallery

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  IHC staining of human tonsillitis using 66801-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66801-1-Ig (CCR6 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 66801-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66801-1-Ig (CCR6 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human tonsillitis using 66801-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CCR6 antibody (66801-1-Ig, Clone: 1C11A9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: human tonsillitis tissue

Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF): IF : 1:400-1:1600

Sample-dependent, check data in validation data gallery

Product Information

66801-1-Ig targets CCR6 in IHC, IF applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: CCR6 fusion protein Ag24195
• Full Name: chemokine (C-C motif) receptor 6
• Calculated molecular weight: 42 kDa
• GenBank accession number: BC037960
• Gene symbol: CCR6
• Gene ID (NCBI): 1235
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CCR6, the gene encoding chemokine (C-C motif) receptor 6, that was a specific marker for Th17 cells, distinguishing them from other helper T-cells. Although CCR6 also expressed in IL-17-producing γδ T cells as well as in subsets of B cells and dendritic cells (PMID: 19830744 ). CCR6 polymorphism was associated with rheumatoid arthritis susceptibility (PMID: 20453841). CD196 is involved in host defense and inflammation at epithelial sites and in IL-17-driven autoimmunity in human diseases.