CCR6 Monoclonal antibody

CCR6 Monoclonal Antibody for IF, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1C11A9

Cat no : 66801-1-Ig

Synonyms

BN 1, C C chemokine receptor type 6, C C CKR 6, CC CKR 6, CCR 6, CCR6, CD196, Chemokine receptor like 3, CKR L3, CKR6, CKRL3, CMKBR6, DCR2, DRY 6, DRY6, G protein coupled receptor 29, GPR CY4, GPR29, GPRCY4, LARC receptor, STRL22



Tested Applications

Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:400-1:1600
Sample-dependent, check data in validation data gallery

Product Information

66801-1-Ig targets CCR6 in IHC, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CCR6 fusion protein Ag24195
Full Name chemokine (C-C motif) receptor 6
Calculated molecular weight 42 kDa
GenBank accession numberBC037960
Gene symbol CCR6
Gene ID (NCBI) 1235
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CCR6, also known CD196, is a C-C chemokine receptor that belongs to family A of G protein-coupled receptor superfamily. It is expressed on most B cells, memory T cells, and some subsets of dendritic cells (PMID: 17057190). CCR6 is a receptor for the C-C type chemokine CCL20 (PMID: 9169459). The ligand-receptor pair CCL20-CCR6 is responsible for the chemoattraction of immature dendritic cells, effector/memory T-cells and B-cells and plays a role at skin and mucosal surfaces under homeostatic and inflammatory conditions, as well as in pathology, including cancer and rheumatoid arthritis (PMID: 12948524). CCR6 polymorphism has been associated with rheumatoid arthritis susceptibility and CCR6 is involved in IL-17-driven autoimmunity in human diseases (PMID: 20453841).

Protocols

Product Specific Protocols
IHC protocol for CCR6 antibody 66801-1-IgDownload protocol
IF protocol for CCR6 antibody 66801-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols