Various lysates were subjected to SDS PAGE followed by western blot with 66305-1-Ig (Vinculin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66305-1-Ig (Vinculin Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66305-1-Ig (Vinculin Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (10% Formaldehyde ) fixed HeLa cells using 66305-1-Ig(Vinculin antibody) at dilution of 1:200 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

1X10^6 HeLa cells were stained with 0.2ug Vinculin antibody (66305-1-Ig, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1500.

Various lysates were subjected to SDS PAGE followed by western blot with 26520-1-AP (Vinculin antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 26520-1-AP (Vinculin antibody) at dilution of 1:600 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL555-phalloidine stains F-actin (red).

Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 26520-1-AP (Vinculin Antibody) at dilution of 1:200 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 26520-1-AP (Vinculin Antibody) at dilution of 1:200 (under 40x lens).

1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human Vinculin (26520-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite® Plus 488 Vinculin antibody (CL488-26520) at dilution of 1:200, CL594-Phalloidin (red).

Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CoraLite®594 Vinculin antibody (CL594-26520) at dilution of 1:400, CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red).

Vinculin fusion protein Ag24942 SDS-PAGE

Vinculin fusion protein Ag24946 SDS-PAGE

Vinculin fusion protein Ag3705 SDS-PAGE

Raji cells were subjected to SDS PAGE followed by western blot with 23288-1-AP (TRIF/TICAM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 23288-1-AP (TRIF/TICAM1 antibody) at dilution of 1:200 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 23288-1-AP (TRIF/TICAM1 antibody) at dilution of 1:200 (under 40x lens).