Multiplexing

Notes on Multiplexing with the FlexAble Antibody Labeling Kits

Step 1-4 see "Standard Workflow"

Primary antibodies of different species can be combined in the same tube and and labeled simultaneously using corresponding species-specific FlexLinkers. Scale up the reagent volumes appropriately based on the amount of primary antibody to be labeled.

For primary antibodies of the same species, label each antibody individually. Once the primary antibodies are labeled, combine and use in your desired application.

Antibodies labeled with the FlexAble kit can be used  alongside pre-conjugated primary antibodies.