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|Citrate Buffer||For 1000 ml||1x TBS||For 1000 ml|
|10 mM Trisodium citrate ₂H₂O||2.9 g||20 mM Tris-base||2.4 g|
|1.9 mM citric acid H₂O||0.4 g||150 mM NaCl||8.7 g|
|Adjust pH to 6.0||Adjust pH to 7.6|
|Add ddH₂O to 1000 ml||Add ddH₂O to 1000 ml|
Tissue sections should be placed on a glass slide. Allow adhesion of tissue to slide by air drying and additional baking in an incubator. Cut and unbaked slides can be stored at 4°C for later use. However, storage might have an effect on the antigenic potential and this varies for each protein. Therefore, it is recommended to minimize storage time or to use freshly prepared slides.