Culturing Kidney Organoids: Best Practices for Differentiation and Long-Term Maintenance

Kidney organoids have emerged as powerful in vitro systems for modeling development, studying disease mechanisms, and advancing regenerative medicine. However, generating and culturing these 3D models can be challenging, requiring careful optimization of differentiation protocols and culture conditions.

Dr. Juliar will cover:

• Workflow overview for stem cell differentiation into kidney organoids
• Tips for optimizing robust organoid formation, including considerations for media
• Best practices for maintaining organoids over extended culture periods
• Characterization of organoid morphology and functional maturity
• Troubleshooting tips for overcoming variability and low viability