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PPP3CC-specific Polyclonal antibody

PPP3CC-specific Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 55163-1-AP

Synonyms

36 53aa, CALNA3, CAM PRP catalytic subunit, CNA3, PPP3CC

Validation Data Gallery

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  • Western Blot (WB) analysis of fetal human brain tissue using PPP3CC-specific Polyclonal antibody (55163-1-AP)

    WB analysis of fetal human brain using 55163-1-AP

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 55163-1-AP (PPP3CC-specific antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using PPP3CC-specific Polyclonal antibody (55163-1-AP)

    WB analysis of human testis using 55163-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 55163-1-AP (PPP3CC-specific antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using PPP3CC-specific Polyclonal antibody (55163-1-AP)

    WB analysis of human brain using 55163-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 55163-1-AP (PPP3CC-specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using PPP3CC-specific Polyclonal antibody (55163-1-AP)

    WB analysis of human brain using 55163-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 55163-1-AP (PPP3CC-specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using PPP3CC-specific Polyclonal antibody (55163-1-AP)

    WB analysis of human brain using 55163-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 55163-1-AP (PPP3CC-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using PPP3CC-specific Polyclonal antibody (55163-1-AP)

    IF Staining of U2OS using 55163-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using 55163-1-AP (PPP3CC-specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected infetal human brain tissue, human brain tissue, human testis tissue
Positive IF detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

55163-1-AP targets PPP3CC-specific in WB, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name protein phosphatase 3 (formerly 2B), catalytic subunit, gamma isoform
Calculated Molecular Weight 58 kDa
Observed Molecular Weight 58 kDa
GenBank Accession NumberNM_005605
Gene Symbol PPP3CC
Gene ID (NCBI) 5533
RRIDAB_10860906
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PPP3CC, also named as CALNA3 and CNA3, belongs to the PPP phosphatase family and PP-2B subfamily. It is a calcium-dependent, calmodulin-stimulated protein phosphatase. It may have a role in the calmodulin activation of calcineurin. This antibody is specific to PPP3CC.

Protocols

Product Specific Protocols
WB protocol for PPP3CC-specific antibody 55163-1-APDownload protocol
IF protocol for PPP3CC-specific antibody 55163-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Acta Biomater

Substrate stiffness regulates differentiation of induced pluripotent stem cells into heart valve endothelial cells.

Authors - Hong Cao
View Article