alpha Actin Antibody

alpha Actin Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 23660-1-AP

Synonyms

ACTA, ACTA1, ACTA1 skeletal Muscle Actin, actin, Actin, alpha skeletal muscle, Alpha actin 1, ASMA, CFTD, α actin



Tested Applications

Positive WB detected inrat skeletal muscle tissue, mouse skeletal muscle tissue
Positive IP detected inmouse skeletal muscle tissue
Positive IHC detected inhuman colon tissue, human heart tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:8000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The 23660-1-AP immunogen is Fusion Protein alpha Actin protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen alpha Actin fusion protein Ag19151
Full Name actin, alpha 1, skeletal muscle
Calculated molecular weight 42 kDa
Observed molecular weight 40-42 kDa
GenBank accession number BC012597
Gene symbol ACTA1
Gene ID (NCBI) 58
Conjugate Unconjugated
Form Liquid
Purification Method Antigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for alpha Actin antibody 23660-1-APDownload protocol
IHC protocol for alpha Actin antibody 23660-1-APDownload protocol
IF protocol for alpha Actin antibody 23660-1-APDownload protocol
IP protocol for alpha Actin antibody 23660-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIF

Mol Med Rep

Function of BRD4 in the pathogenesis of high glucose‑induced cardiac hypertrophy.

Authors - Qian Wang
humanWB

Acta Biochim Biophys Sin (Shanghai)

Epigallocatechin-3-gallate inhibits angiotensin II-induced cardiomyocyte hypertrophy via regulating Hippo signaling pathway in H9c2 rat cardiomyocytes.

Authors - Yuan Ma
humanWB

Mol Ther Nucleic Acids

lncRNA TUG1 Promotes Cisplatin Resistance by Regulating CCND2 via Epigenetically Silencing miR-194-5p in Bladder Cancer.

Authors - Gan Yu
mouseWB

Cell Death Dis

Long noncoding RNA Neat1 modulates myogenesis by recruiting Ezh2.

Authors - Shanshan Wang
mouseWB

Cell Death Dis

Ginsenoside Rg3 promotes regression from hepatic fibrosis through reducing inflammation-mediated autophagy signaling pathway.

Authors - Xiangxiang Liu
humanWB

Exp Lung Res

The roles of dipeptidyl peptidase-4 and its inhibitors in the regulation of airway epithelial-mesenchymal transition.

Authors - Jingyi Sun