Fetuin-A Monoclonal antibody

Fetuin-A Monoclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1F6B9

Cat no : 66094-1-Ig

Synonyms

A2HS, AHS, AHSG, alpha 2 HS glycoprotein, Alpha 2 Z globulin, Ba alpha 2 glycoprotein, FETUA, Fetuin A, HSGA



Tested Applications

Positive WB detected inHepg2 cells, human heart tissue, human plasma tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:6000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 66094-1-Ig is Fetuin-A Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Fetuin-A fusion protein Ag10073
Full Name alpha-2-HS-glycoprotein
Calculated molecular weight 367 aa, 39 kDa
Observed molecular weight 58 kDa
GenBank accession numberBC048198
Gene symbol AHSG
Gene ID (NCBI) 197
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Fetuin-A (alpha-2-HS-glycoprotein, AHSG), a liver borne plasma protein, contributes to the prevention of soft tissue calcification, modulates inflammation, reduces INS sensitivity and fosters weight gain following high fat diet or ageing. It is involved in several functions, such as endocytosis, brain development and the formation of bone tissue. The protein is commonly present in the cortical plate of the immature cerebral cortex and bone marrow hemopoietic matrix, and it has therefore been postulated that it participates in the development of the tissues.

Protocols

Product Specific Protocols
WB protocol for Fetuin-A antibody 66094-1-IgDownload protocol
IHC protocol for Fetuin-A antibody 66094-1-IgDownload protocol
IF protocol for Fetuin-A antibody 66094-1-IgDownload protocol
IP protocol for Fetuin-A antibody 66094-1-IgDownload protocol
FC protocol for Fetuin-A antibody 66094-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols