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ANP32B Antibody

ANP32B Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

5A1F12

Cat no : 66160-1-Ig

Synonyms

ANP32B, APRIL, PHAPI2, Silver stainable protein SSP29, SSP29



Tested Applications

Positive WB detected inLNCaP cells, mouse brain tissue, fetal human brain tissue, HEK-293 cells, rat testis tissue, HeLa cells, HCT 116 cells, A431 cells, HEK-293 cells, U2OS cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66160-1-Ig targets ANP32B in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen ANP32B fusion protein Ag1274
Full Name acidic (leucine-rich) nuclear phosphoprotein 32 family, member B
Calculated molecular weight 29 kDa
Observed molecular weight 29 kDa
GenBank accession number BC013003
Gene symbol ANP32B
Gene ID (NCBI) 10541
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ANP32B is a member of the highly conserved acidic leucine-rich nuclear phosphoprotein 32 (ANP32) family. The ANP32 proteins have been implicated in a broad array of physiological processes, including cell differentiation, apoptotic cell death, and cell proliferation. ANP32B was identified as a novel direct substrate for caspase-3 and acted as a negative regulator for leukemic cell apoptosis. ANP32B controls the expression of the dendritic cell maturation factor CD83 by regulating the transport of its mRNA to the cytoplasm. It also modulates the activity of the transcription factor Kruppel-like factor 5 (KLF5).

Protocols

Product Specific Protocols
WB protocol for ANP32B antibody 66160-1-IgDownload protocol
IF protocol for ANP32B antibody 66160-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Virol

ANP32 proteins are essential for influenza virus replication in human cells.

Authors - Ecco Staller
  • KO Validated