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  • Featured Product
  • KD/KO Validated

Amphiregulin Polyclonal antibody

Amphiregulin Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 16036-1-AP

Synonyms

amphiregulin, AR, AREG, AREGB, CRDGF, SDGF

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using Amphiregulin Polyclonal antibody (16036-1-AP)

    WB analysis of MCF-7 using 16036-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 16036-1-AP (Amphiregulin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using Amphiregulin Polyclonal antibody (16036-1-AP)

    WB analysis of A549 using 16036-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 16036-1-AP (Amphiregulin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using Amphiregulin Polyclonal antibody (16036-1-AP)

    WB analysis of HepG2 using 16036-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16036-1-AP (Amphiregulin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using Amphiregulin Polyclonal antibody (16036-1-AP)

    WB analysis of MCF-7 using 16036-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 16036-1-AP (Amphiregulin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using Amphiregulin Polyclonal antibody (16036-1-AP)

    IP experiment of HepG2 using 16036-1-AP

    IP result of anti-Amphiregulin (IP:16036-1-AP, 4ug; Detection:16036-1-AP 1:2000) with HepG2 cells lysate 3600ug.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Amphiregulin Polyclonal antibody (16036-1-AP)

    IHC staining of human colon cancer using 16036-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16036-1-AP (Amphiregulin antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Amphiregulin Polyclonal antibody (16036-1-AP)

    IHC staining of human colon cancer using 16036-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16036-1-AP (Amphiregulin antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using Amphiregulin Polyclonal antibody (16036-1-AP)

    IHC staining of human colon using 16036-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16036-1-AP (Amphiregulin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human colon cancer tissue using Amphiregulin Polyclonal antibody (16036-1-AP)

    IF Staining of human colon cancer using 16036-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Amphiregulin antibody (16036-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human colon cancer tissue using Amphiregulin Polyclonal antibody (16036-1-AP)

    IF Staining of human colon cancer using 16036-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Amphiregulin antibody (16036-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of HeLa cells using Amphiregulin Polyclonal antibody (16036-1-AP)

    FC experiment of HeLa using 16036-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human Amphiregulin (16036-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inMCF-7 cells, A549 cells, HepG2 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman colon cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman colon cancer tissue
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16036-1-AP targets Amphiregulin in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Amphiregulin fusion protein Ag8907
Full Name amphiregulin
Calculated Molecular Weight 252 aa, 28 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberBC009799
Gene Symbol AREG
Gene ID (NCBI) 374
RRIDAB_2227602
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Amphiregulin (AREG) is one of the ligands of the epidermal growth factor receptor (EGFR). AREG plays a central role in mammary gland development and branching morphogenesis in organs and is expressed both in physiological and in cancerous tissues. The AREG protein is synthesized as a 252-amino acid transmembrane precursor, pro-AREG. At the plasma membrane, pro-AREG is subjected to sequential proteolytic cleavages within its ectodomain and is then released as the soluble AREG protein. Depending on the cell type and microenvironment, AREG can be produced in multiple cellular and mature forms using alternative pro-AREG cleavage sites and glycosylation motifs. Post-translastional modfications of 50-kDa pro-AREG produces a major soluble 43-kDa form, 28-, 26-, 16-kDa membrane anchored forms, and soluble 21-, 19-, and 9-kDa forms (PMID: 9642297).

Protocols

Product Specific Protocols
WB protocol for Amphiregulin antibody 16036-1-APDownload protocol
IHC protocol for Amphiregulin antibody 16036-1-APDownload protocol
IF protocol for Amphiregulin antibody 16036-1-APDownload protocol
IP protocol for Amphiregulin antibody 16036-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Res

CD317 Activates EGFR by Regulating Its Association with Lipid Rafts.

Authors - Guizhong Zhang
View Article
humanIP

Proc Natl Acad Sci U S A

AhR controls redox homeostasis and shapes the tumor microenvironment in BRCA1-associated breast cancer.

Authors - Shawn P Kubli
View Article
humanWB

Cell Biosci

TPM2 attenuates progression of prostate cancer by blocking PDLIM7-mediated nuclear translocation of YAP1

Authors - Zonglong Wu
View Article
humanWB, IF

Oncogene

The EGF receptor ligand amphiregulin controls cell division via FoxM1.

Authors - S W Stoll
View Article
humanWB,IHC,IP

Aging Cell

Targeting amphiregulin (AREG) derived from senescent stromal cells diminishes cancer resistance and averts programmed cell death 1 ligand (PD-L1)-mediated immunosuppression.

Authors - Qixia Xu
  • KD Validated
View Article
humanWB,IF

J Invest Dermatol

Membrane-Tethered Intracellular Domain of Amphiregulin Promotes Keratinocyte Proliferation.

Authors - Stefan W Stoll
View Article