Tested Applications
| Positive WB detected in | mouse cerebellum tissue, rat cerebellum tissue, rat kidney tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84863-5-RR targets ACBP/DBI in WB, ELISA applications and shows reactivity with mouse, rat samples.
| Tested Reactivity | mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg2462 Product name: Recombinant Mouse ACBP/DBI protein (rFc Tag) (HPLC verified) Source: mammalian cells-derived, pHZ-KIsec-N-rFc Tag: N-rFc Domain: 1-87 aa of NM_007830.4 Sequence: MSQAEFDKAAEEVKRLKTQPTDEEMLFIYSHFKQATVGDVNTDRPGLLDLKGKAKWDSWNKLKGTSKESAMKTYVEKVDELKKKYGI Predict reactive species |
| Full Name | diazepam binding inhibitor |
| Calculated Molecular Weight | 10KDa |
| Observed Molecular Weight | 10 kDa |
| GenBank Accession Number | NM_007830.4 |
| Gene Symbol | Dbi |
| Gene ID (NCBI) | 13167 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | P31786 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Acbp/Dbi (acyl-CoA binding protein/Diazepam-binding inhibitor) is a widely expressed protein that binds long-chain fatty acyl-CoA esters and plays a role in fatty acyl-CoA transport and pool formation. Acbp/Dbi is critical to cellular proliferation, differentiation, mitochondrial functions, and autophagy. Deletion of Acbp in mouse results in sebocyte hyperplasia and sparse, matted hair with a greasy appearance (PMID: 32632162, 16902415).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ACBP/DBI antibody 84863-5-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
