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Beta-2-microglobulin Antibody 0 Publications

Mouse Monoclonal| Catalog number: 66207-1-Ig |CloneNo.: 1C3B7

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Con: 219 μg/150 μl

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Tested Applications

Applications:
WB, IHC, ELISA
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Images See all

Beta-2-microglobulin Antibody WB Raji cells 66207-1-Ig

Raji cells were subjected to SDS PAGE followed by western blot with 66207-1-Ig( B2M Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours

Beta-2-microglobulin Antibody IHC human prostate cancer tissue 66207-1-Ig

Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66207-1-Ig (B2M Antibody) at dilution of 1:200 (under 10x lens)

Beta-2-microglobulin Antibody IHC human prostate cancer tissue 66207-1-Ig

Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66207-1-Ig (B2M Antibody) at dilution of 1:200 (under 40x lens)

Species specificity:
human
Positive WB detected in:
Raji cells
Positive IHC detected in:
human prostate cancer tissue, human renal cell carcinoma tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:1000-1:8000
IHC : 1:20-1:200
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Mouse
Purification method:
Protein A purification
Isotype:
IgG2b
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Peptide
Full name:
beta-2-microglobulin
Calculated molecular weight:
14 kDa
Observed molecular weight:
13 kDa
GenBank accession number:
BC032589
Gene ID (NCBI):
567
Gene symbol
B2M
Synonyms
B2M, Beta-2-microglobulin, HDCMA22P

Background

Beta-2-microglobulin (B2M) is a component of MHC class I molecules, which are present on the surface of nearly all nucleated cells. It can be found in body fluids under physiologic conditions as a result of shedding from cell surfaces or intracellular release. B2M has various biological functions, including antigen presentation. Investigations reveal that increased synthesis and release of B2M are present in several malignant diseases.

Customer Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Share your scientific insight on this antibody

Beta-2-microglobulin Mouse Monoclonal antibody

Santhosh (Verified Customer), 04-15-2019
Applications
Immunohistochemistry,
Primary Antibody Dilution
1:200
Beta-2-microglobulin Antibody Immunohistochemistry, validation (1:200 dilution) in (Cat no:66207-1-Ig)

Beta-2-microglobulin Antibody Immunohistochemistry, validation (1:200 dilution) in (Cat no:66207-1-Ig)

Lot Number
Human tonsil FFPE tissue rehydrated and antigen retrieval performed with citrate buffer.

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