Beta-2-microglobulin Monoclonal Antibody for IHC, WB, ELISA
Host / Isotype
Mouse / IgG2b
IHC, WB, ELISA
Cat no : 66207-1-Ig
B2M, Beta-2-microglobulin, HDCMA22P
|Positive WB detected in||Raji cells|
|Positive IHC detected in||human prostate cancer tissue, human renal cell carcinoma tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:8000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
66207-1-Ig targets Beta-2-microglobulin in IHC, WB, ELISA applications and shows reactivity with human samples.
|Host / Isotype||Mouse / IgG2b|
|Calculated molecular weight||14 kDa|
|Observed molecular weight||13 kDa|
|GenBank accession number||BC032589|
|Gene ID (NCBI)||567|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20oC. Aliquoting is unnecessary for -20oC storage.|
Beta-2-microglobulin (B2M) is a component of MHC class I molecules, which are present on the surface of nearly all nucleated cells. It can be found in body fluids under physiologic conditions as a result of shedding from cell surfaces or intracellular release. B2M has various biological functions, including antigen presentation. Investigations reveal that increased synthesis and release of B2M are present in several malignant diseases.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Santhosh Sivajothi (04-15-2019)
Human tonsil FFPE tissue rehydrated and antigen retrieval performed with citrate buffer.