CoraLite®594-conjugated Beta-2-Microglobulin Monoclonal antibody

Beta-2-Microglobulin Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1C3B7

Cat no : CL594-66207

Synonyms

B2M, beta 2 microglobulin, Beta-2-Microglobulin, HDCMA22P

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Validation Data Gallery

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  IF Staining of human prostate cancer using CL594-66207

  Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using CoraLite®594 Beta-2-Microglobulin antibody (CL594-66207, Clone: 1C3B7 ) at dilution of 1:200.

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  IF Staining of human prostate cancer using CL594-66207

  Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using CoraLite®594 Beta-2-Microglobulin antibody (CL594-66207, Clone: 1C3B7 ) at dilution of 1:100.

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Tested Applications

• Positive IF detected in: human prostate cancer tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66207 targets Beta-2-Microglobulin in IF applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: beta-2-microglobulin
• Calculated Molecular Weight: 14 kDa
• Observed Molecular Weight: 13 kDa
• GenBank Accession Number: BC032589
• Gene Symbol: B2M
• Gene ID (NCBI): 567
• RRID: AB_2919964
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Beta-2-microglobulin (B2M) is a component of MHC class I molecules, which are present on the surface of nearly all nucleated cells. It can be found in body fluids under physiologic conditions as a result of shedding from cell surfaces or intracellular release. B2M has various biological functions, including antigen presentation. Investigations reveal that increased synthesis and release of B2M are present in several malignant diseases.