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  • KD/KO Validated

BAP31 Antibody

BAP31 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 11200-1-AP

Synonyms

6C6 AG, BAP31, BCAP31, CDM, DXS1357E, p28, Protein CDM



Tested Applications

Positive WB detected inHEK-293 cells, A431 cells, HeLa cells, human placenta tissue, mouse placenta tissue
Positive IP detected inA431 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

11200-1-AP targets BAP31 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BAP31 fusion protein Ag1668
Full Name B-cell receptor-associated protein 31
Calculated molecular weight 28 kDa
Observed molecular weight 28 kDa
GenBank accession number BC014323
Gene symbol BCAP31
Gene ID (NCBI) 10134
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BAP31 is a chaperone protein that is abundant in the endoplasmic reticulum (ER). BAP31 plays a role in the export of secreted proteins in the ER, the recognition of abnormally folded protein and their targeting to the ER associated-degradation (ERAD). It also serves as a cargo receptor for the export of transmembrane proteins. BAP31 may be involved in apoptosis.

Protocols

Product Specific Protocols
WB protocol for BAP31 antibody 11200-1-APDownload protocol
IHC protocol for BAP31 antibody 11200-1-APDownload protocol
IF protocol for BAP31 antibody 11200-1-APDownload protocol
IP protocol for BAP31 antibody 11200-1-APDownload protocol
FC protocol for BAP31 antibody 11200-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Cell Death Dis

MiR-451a suppressing BAP31 can inhibit proliferation and increase apoptosis through inducing ER stress in colorectal cancer.

Authors - Ke Xu
  • KD Validated
mouseWB

Res Vet Sci

Proteomic analyses reveal that Orf virus induces the activation and maturation of mouse bone marrow-derived dendritic cells.

Authors - Qian Feng
humanWB,IF

J Biol Chem

Down-regulation of the islet-specific zinc transporter-8 (ZnT8) protects human insulinoma cells against inflammatory stress.

Authors - Chengfeng Merriman
humanWB

J Cell Sci

Expansion of DUB functionality by alternative isoforms: USP35, a case study.

Authors - Pawel Leznicki
humanWB,IP

EMBO J

Fis1 and Bap31 bridge the mitochondria-ER interface to establish a platform for apoptosis induction.

Authors - Iwasawa Ryota R
  • KD Validated
humanWB

EMBO J

FUNDC1 regulates mitochondrial dynamics at the ER-mitochondrial contact site under hypoxic conditions.

Authors - Wenxian Wu