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  • KD/KO Validated

TGFBI / BIGH3 Monoclonal antibody

TGFBI / BIGH3 Monoclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3E11D11

Cat no : 60007-1-Ig

Synonyms

Beta ig h3, BIGH3, CDB1, CDG2, CDGG1, CSD, CSD1, CSD2, CSD3, EBMD, Kerato epithelin, LCD1, RGD CAP, TGFBI



Tested Applications

Positive WB detected inhuman kidney tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue, human skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

60007-1-Ig targets TGFBI / BIGH3 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen TGFBI / BIGH3 fusion protein Ag0241
Full Name transforming growth factor, beta-induced, 68kDa
Calculated molecular weight 683 aa, 75 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC000097
Gene symbol TGFBI
Gene ID (NCBI) 7045
Conjugate Unconjugated
Form Liquid
Purification Method Caprylic acid/ammonium sulfate precipitation
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TGFBI, also named as BIGH3, Kerato-epithelin and RGD-CAP, binds to type I, II, and IV collagens. TGFBI is an adhesion protein which may play an important role in cell-collagen interactions. In cartilage, it may be involved in endochondral bone formation. TGFBI is an extracellular matrix adaptor protein, it has been reported to be differentially expressed in transformed tissues. TGFBI is a predictive factor of the response to chemotherapy, and suggest the use of TGFBI-derived peptides as possible therapeutic adjuvants for the enhancement of responses to chemotherapy.(PMID:20509890) Defects in TGFBI are the cause of epithelial basement membrane corneal dystrophy (EBMD). Defects in TGFBI are the cause of corneal dystrophy Groenouw type 1 (CDGG1). Defects in TGFBI are the cause of corneal dystrophy lattice type 1 (CDL1). Defects in TGFBI are a cause of corneal dystrophy Thiel-Behnke type (CDTB). Defects in TGFBI are the cause of Reis-Buecklers corneal dystrophy (CDRB). Defects in TGFBI are the cause of lattice corneal dystrophy type 3A (CDL3A). Defects in TGFBI are the cause of Avellino corneal dystrophy (ACD).

Protocols

Product Specific Protocols
WB protocol for TGFBI / BIGH3 antibody 60007-1-IgDownload protocol
IHC protocol for TGFBI / BIGH3 antibody 60007-1-IgDownload protocol
IP protocol for TGFBI / BIGH3 antibody 60007-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Curr Eye Res

Modified BIGH3 with an RGDRGD motif promotes human corneal epithelial cell adhesion and migration in vitro.

Authors - Ge Hongyan H
humanWB

PLoS One

Absence of Vitamin K-Dependent γ-Carboxylation in Human Periostin Extracted from Fibrotic Lung or Secreted from a Cell Line Engineered to Optimize γ-Carboxylation.

Authors - Douglas S Annis
humanWB,IHC

Neoplasia

BIGH3 Promotes Osteolytic Lesions in Renal Cell Carcinoma Bone Metastasis by Inhibiting Osteoblast Differentiation.

Authors - Tianhong Pan
  • KD Validated
humanWB

Cell Mol Life Sci

TGFbeta-induced protein mediates lymphatic endothelial cell adhesion to the extracellular matrix under low oxygen conditions.

Authors - Irigoyen M M
humanWB,IHC

Int J Cancer

Transforming growth factor-beta-induced protein secreted by peritoneal cells increases the metastatic potential of ovarian cancer cells.

Authors - Ween Miranda P MP