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BZW1 Polyclonal antibody

BZW1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 19917-1-AP

Synonyms

BZAP45, BZW1, KIAA0005, Nbla10236, Protein Orf

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using BZW1 Polyclonal antibody (19917-1-AP)

    WB analysis of MCF-7 using 19917-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 19917-1-AP (BZW1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using BZW1 Polyclonal antibody (19917-1-AP)

    WB analysis of HeLa using 19917-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 19917-1-AP (BZW1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using BZW1 Polyclonal antibody (19917-1-AP)

    IHC staining of human testis using 19917-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 19917-1-AP (BZW1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using BZW1 Polyclonal antibody (19917-1-AP)

    IHC staining of human testis using 19917-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 19917-1-AP (BZW1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using BZW1 Polyclonal antibody (19917-1-AP)

    IHC staining of human lung cancer using 19917-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 19917-1-AP (BZW1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using BZW1 Polyclonal antibody (19917-1-AP)

    IHC staining of human lung cancer using 19917-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 19917-1-AP (BZW1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using BZW1 Polyclonal antibody (19917-1-AP)

    IF Staining of HeLa using 19917-1-AP

    Immunofluorescent analysis of Hela cells, using BZW1 antibody 19917-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inMCF7 cells, HeLa cells
Positive IHC detected inhuman testis tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19917-1-AP targets BZW1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BZW1 fusion protein Ag13830
Full Name basic leucine zipper and W2 domains 1
Calculated Molecular Weight 353 aa, 41 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC001804
Gene Symbol BZW1
Gene ID (NCBI) 9689
RRIDAB_10693530
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for BZW1 antibody 19917-1-APDownload protocol
IHC protocol for BZW1 antibody 19917-1-APDownload protocol
IF protocol for BZW1 antibody 19917-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Leukemia

IPO11 regulates the nuclear import of BZW1/2 and is necessary for AML cells and stem cells.

Authors - Boaz Nachmias
View Article
human,mouseWB,IHC,IF

Genomics

BZW1 as an oncogene is associated with patient prognosis and the immune microenvironment in glioma

Authors - Linyao Zhao
  • KD Validated
View Article