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Bcl2 Antibody

Bcl2 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat, human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 26593-1-AP

Synonyms

AW986256, B cell leukemia/lymphoma 2, Bcl2, Bcl-2, C430015F12Rik, D630044D05Rik, D830018M01Rik



Tested Applications

Positive WB detected inJurkat cells, C2C12 cells, HL-60 cells, mouse kidney tissue, rat spleen tissue, mouse testis tissue, mouse heart tissue, NIH/3T3 cells, THP-1 cells, rat spleen tissue
Positive IHC detected inhuman tonsillitis tissue, mouse spleen tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The 26593-1-AP immunogen is Fusion Protein Bcl2 protein expressed in E. coli.

Reactivity mouse, rat, human
Cited Species chicken, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Bcl2 fusion protein Ag24260
Full Name B-cell leukemia/lymphoma 2
Calculated molecular weight 26 kDa
Observed molecular weight 26 kDa
GenBank accession number NM-009741
Gene symbol Bcl2
Gene ID (NCBI) 12043
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

1.       What is Bcl-2?

Bcl-2 (B-cell lymphoma 2) is an outer mitochondrial membrane protein that via heterodimerization with BAX proteins controls apoptosis. Abnormalities of Bcl-2 activity can lead to cancer, autoimmune diseases, and schizophrenia.

2.       FAQs for Bcl-2

  1.  How to measure Bcl-2 and Bax apoptotic markers by western blotting

    Generally, Bcl-2 protein is considered to have anti-apoptotic activity, while Bax has apoptotic activity. Comparing the ratio between Bcl-2 and Bax protein levels in different samples or cells subjected to treatments can reflect the apoptotic state of the cells. Expression of Bax can vary between cell lines and it is important to compare it to Bcl-2 levels.

  2. What band size should I expect in western blotting for Bcl-2?

    Generally, Bcl-2 protein is considered to have anti-apoptotic activity, while Bax has apoptotic activity. Comparing the ratio between Bcl-2 and       Bax protein levels in different samples or cells subjected to treatments can reflect the apoptotic state of the cells. Expression of Bax can vary between cell lines and it is important to compare it to Bcl-2 levels.


Protocols

Product Specific Protocols
WB protocol for Bcl2 antibody 26593-1-APDownload protocol
IHC protocol for Bcl2 antibody 26593-1-APDownload protocol
IF protocol for Bcl2 antibody 26593-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Acta Cir Bras

Milk fat globule-epidermal growth factor 8 (MFG-E8) attenuates sepsis-induced acute kidney injury by inhibiting NF-κB signaling pathway1.

Authors - Yang Zhao
mouseWB

Acta Pharmacol Sin

Protective effects of specific cannabinoid receptor 2 agonist GW405833 on concanavalin A-induced acute liver injury in mice.

Authors - Ze-Bing Huang
ratWB

Pain Physician

Toxicity Effects of Methylene Blue on Rat Intervertebral Disc Annulus Fibrosus Cells.

Authors - Liang Zhang
ratIHC

Ecotoxicol Environ Saf

Bone marrow mesenchymal stem cells repair Cr (VI)- injured kidney by regulating mitochondria-mediated apoptosis and mitophagy mediated via the MAPK signaling pathway.

Authors - Fei Yin
ratWB

Aging (Albany NY)

H2S restores the cardioprotective effects of ischemic post-conditioning by upregulating HB-EGF/EGFR signaling.

Authors - Yuanzhou Zhang
mouseWB

Life Sci

Dihydromyricetin alleviates acetaminophen-induced liver injury via the regulation of transformation, lipid homeostasis, cell death and regeneration.

Authors - Sijing Dong