CD34 Monoclonal antibody
CD34 Monoclonal Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human
Applications
WB, IHC, IF, FC, ELISA
Conjugate
Unconjugated
CloneNo.
3C8G12
Cat no : 60180-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | human placenta tissue, human uterus tissue, human testis tissue |
| Positive IHC detected in | human liver cancer tissue, human placenta tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | human placenta tissue, human liver cancer tissue, human tonsillitis tissue |
| Positive FC detected in | HUVEC cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:2500-1:10000 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IF | See 7 publications below |
| FC | See 4 publications below |
Product Information
60180-1-Ig targets CD34 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CD34 fusion protein Ag5996 |
| Full Name | CD34 molecule |
| Calculated Molecular Weight | 41 kDa |
| Observed Molecular Weight | 105 kDa |
| GenBank Accession Number | BC039146 |
| Gene Symbol | CD34 |
| Gene ID (NCBI) | 947 |
| RRID | AB_10733337 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CD34 is a 105- to 120-kDa glycophosphoprotein expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nerve tissue. CD34 is a commonly used marker for identifying human hematopoietic stem/progenitor cells and mediates cell adhesion and lymphocyte homing by binding L-selectin and E-selectin ligands. CD34 is also one of the best negative selection markers for characterizing and/or isolating human MSCs from bone marrow and other sources. Along with other positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification. The calculated molecular mass of human CD34 is 41 kDa, various forms with different molecular weights may be produced due to different glycosylation patterns and alternative splicing (PMID: 24375067; 15750786).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CD34 antibody 60180-1-Ig | Download protocol |
| IHC protocol for CD34 antibody 60180-1-Ig | Download protocol |
| IF protocol for CD34 antibody 60180-1-Ig | Download protocol |
| FC protocol for CD34 antibody 60180-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Gastroenterology Proteomic characterization identifies clinically relevant subgroups of gastrointestinal stromal tumors | ||
Front Immunol Thrombotic microangiopathy mediates poor prognosis among lupus nephritis via complement lectin and alternative pathway activation | ||
Front Bioeng Biotechnol Primary Repair for Treating Acute Proximal Anterior Cruciate Ligament Tears: A Histological Analysis and Prospective Clinical Trial. | ||
Exp Cell Res Nrf2 activation is involved in osteogenic differentiation of periodontal ligament stem cells under cyclic mechanical stretch. | ||
Biomed Mater The additive effects of photobiomodulation and bioactive glasses on enhancing early angiogenesis. | ||
