Validation Data Gallery
|Positive WB detected in||pig spleen tissue, Raji cells|
|Positive IHC detected in||human tonsillitis tissue, human colon tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||human tonsillitis tissue, mouse spleen tissue|
|Positive FC detected in||Raji cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunohistochemistry (IHC)||IHC : 1:5000-1:20000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
60287-1-Ig targets CD45 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, pig, mouse samples.
|Tested Reactivity||human, pig, mouse|
|Cited Reactivity||mouse, swine|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||CD45 fusion protein Ag13712|
|Full Name||protein tyrosine phosphatase, receptor type, C|
|Calculated molecular weight||1304 aa, 147 kDa|
|Observed molecular weight||220 kDa|
|GenBank accession number||BC014239|
|Gene ID (NCBI)||5788|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Protein tyrosine phosphatase receptor type C (PTPRC) is also known as CD45 antigen, which was originally called leukocyte common antigen (LCA). CD45 is an enzyme required for T-cell activation through the antigen receptor consisting of two intracellular phosphatase domains, a transmembrane domain and an extracellular domain. Upon T-cell activation, CD45 recruits and dephosphorylates SKAP1 via an interaction through the first PTPase domain, which is the only domain to possess intrinsic kinase activity. However, both domains are required for appropriate phosphatase and T-cell antigen receptor activity (PMID: 8076596).
What is the molecular weight of CD45?
The molecular weight of CD45 is a 180-240 kDa.
What are the isoforms of CD45?
Six different human isoforms of CD45 have been isolated, containing all three exons (ABC isoform), two of the three exons (AB and BC isoforms), only one exon (A isoform or B isoform), or no exons (O isoform). All of the isoforms have the same eight amino acids at their amino-terminus (PMID: 12412720).
What is the tissue specificity of CD45?
CD45 is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets (PMID: 7803253).
What are the post-translational modifications of CD45?
CD45 can be heavily N- and O-glycosylated (PMID: 1702721).
What is CD45's involvement in disease?
Defects in CD45 are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID); a severe form of SCID. It is classed by the impairment of both humoral and cell-mediated immunity, leukopenia, and the low or absent levels of antibodies (PMID: 10700239). Also, genetic variants of CD45 have a role in susceptibility to multiple sclerosis (PMID: 12864992).
|Product Specific Protocols|
|WB protocol for CD45 antibody 60287-1-Ig||Download protocol|
|IHC protocol for CD45 antibody 60287-1-Ig||Download protocol|
|IF protocol for CD45 antibody 60287-1-Ig||Download protocol|
|FC protocol for CD45 antibody 60287-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
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