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CD72 Monoclonal antibody

CD72 Monoclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IF, FC, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2C1C6

Cat no : 67424-1-Ig

Synonyms

CD72, CD72 molecule, CD72b, Lyb 2, LYB2

Validation Data Gallery

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  • Western Blot (WB) analysis of Daudi cells using CD72 Monoclonal antibody (67424-1-Ig)

    WB analysis of Daudi using 67424-1-Ig

    Daudi cells were subjected to SDS PAGE followed by western blot with 67424-1-Ig (CD72 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD72 Monoclonal antibody (67424-1-Ig)

    IHC staining of human tonsillitis using 67424-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67424-1-Ig (CD72 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD72 Monoclonal antibody (67424-1-Ig)

    IHC staining of human tonsillitis using 67424-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67424-1-Ig (CD72 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD72 Monoclonal antibody (67424-1-Ig)

    IHC staining of human tonsillitis using 67424-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67424-1-Ig (CD72 antibody) at dilution of 1:1000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of Ramos cells using CD72 Monoclonal antibody (67424-1-Ig)

    IF Staining of Ramos using 67424-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed Ramos cells using CD72 antibody (67424-1-Ig, Clone: 2C1C6 ) at dilution of 1:1600 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD72 Monoclonal antibody (67424-1-Ig)

    IF Staining of human tonsillitis using 67424-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD72 antibody (67424-1-Ig, Clone: 2C1C6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of Ramos cells using CD72 Monoclonal antibody (67424-1-Ig)

    FC experiment of Ramos using 67424-1-Ig

    1X10^6 Ramos cells were intracellularly stained with 0.4 ug Anti-Human CD72 (67424-1-Ig, Clone:2C1C6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inDaudi cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue, Ramos cells
Positive FC detected inRamos cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67424-1-Ig targets CD72 in WB, IF, FC, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CD72 fusion protein Ag14405
Full Name CD72 molecule
Calculated Molecular Weight 359 aa, 40 kDa
Observed Molecular Weight40 kDa
GenBank Accession NumberBC030227
Gene Symbol CD72
Gene ID (NCBI) 971
RRIDAB_2882663
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD72 is a type II transmembrane glycoprotein of 39-45 kDa and is expressed as a disulphide-linked homodimer (PMID: 1711157). CD72 is a pan B-cell marker covering the full range of differentiation from the very early stages of B cells to mature surface Ig+ B cells (PMID: 1384316). It is expressed in all stages of B cell development except plasma cells. CD72 is also expressed by dendritic cells, tissue macrophages in the red pulp of the spleen and von Kupffer cells in the liver (PMID: 8561576; 1384316). CD72 is the ligand for CD5 (PMID: 1711157). CD72 is a negative regulator of BCR signaling (PMID: 9590210). Interaction of CD100 with CD72 strictly tunes the strength of BCR signals and is essential for maintaining immunological homeostasis as well as generating a proper immune response (PMID: 16113236).

Protocols

Product Specific Protocols
WB protocol for CD72 antibody 67424-1-IgDownload protocol
IHC protocol for CD72 antibody 67424-1-IgDownload protocol
IF protocol for CD72 antibody 67424-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols