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CDC6 Monoclonal antibody

CDC6 Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

6F12D2

Cat no : 66021-1-Ig

Synonyms

Cdc18 related protein, CDC18L, CDC6, CDC6 related protein, HsCDC18, HsCDC6, p62(cdc6)

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using human CDC6 Monoclonal antibody (66021-1-Ig)

    WB analysis using 66021-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66021-1-Ig (CDC6 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of ROS1728 cells using CDC6 Monoclonal antibody (66021-1-Ig)

    WB analysis of ROS1728 using 66021-1-Ig

    ROS1728 cells were subjected to SDS PAGE followed by western blot with 66021-1-Ig (CDC6 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using CDC6 Monoclonal antibody (66021-1-Ig)

    WB analysis of NIH/3T3 using 66021-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66021-1-Ig (CDC6 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using CDC6 Monoclonal antibody (66021-1-Ig)

    WB analysis of HeLa using 66021-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66021-1-Ig (CDC6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using human CDC6 Monoclonal antibody (66021-1-Ig)

    IF Staining of HepG2 using 66021-1-Ig

    Immunofluorescent analysis of HepG2 cells using 66021-1-Ig (CDC6 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG.

Tested Applications

Positive WB detected inHeLa cells, NIH/3T3 cells, ROS1728 cells, HEK-293T cells, Jurkat cell, Raji cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66021-1-Ig targets CDC6 in WB, IF, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CDC6 fusion protein Ag17958
Full Name cell division cycle 6 homolog (S. cerevisiae)
Calculated Molecular Weight 560 aa, 63 kDa
Observed Molecular Weight 65 kDa
GenBank Accession NumberBC025232
Gene Symbol CDC6
Gene ID (NCBI) 990
RRIDAB_2881493
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDC6, also named as CDC18L and HsCDC18, belongs to the CDC6/cdc18 family. It is involved in the initiation of DNA replication and functions as a regulator at the early steps of DNA replication. It also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Defects in CDC6 are the cause of Meier-Gorlin syndrome type 5 (MGORS5). MGORS5 is a syndrome characterized by bilateral microtia, aplasia/hypoplasia of the patellae, and severe intrauterine and postnatal growth retardation with short stature and poor weight gain. This antibody is a mouse monoclonal antibody raised against residues near the C terminus of human CDC6.

Protocols

Product Specific Protocols
WB protocol for CDC6 antibody 66021-1-IgDownload protocol
IF protocol for CDC6 antibody 66021-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols