Ceruloplasmin Polyclonal antibody

Ceruloplasmin Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 21131-1-AP

Synonyms

Ceruloplasmin, ceruloplasmin (ferroxidase), CP, CP 2, Ferroxidase



Tested Applications

Positive WB detected inhuman blood tissue, mouse brain tissue, human plasma tissue
Positive IHC detected inhuman hepatocirrhosis tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21131-1-AP is Ceruloplasmin Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Ceruloplasmin fusion protein Ag15535
Full Name ceruloplasmin (ferroxidase)
Calculated Molecular Weight 1065 aa, 122 kDa
Observed Molecular Weight 130-140 kDa, 85 kDa
GenBank Accession NumberBC146663
Gene Symbol CP
Gene ID (NCBI) 1356
RRIDAB_2878815
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ceruloplasmin (CP) is a serum glycoprotein synthesized by hepatocytes and functions as the major transport protein of copper in blood where it acts as ferrodoxidase and is required for iron transport of transferrin. The intact ceruloplasmin migrated around 130-140 kDa and some proteolytic cleaved fragments could also be observed.

Protocols

Product Specific Protocols
WB protocol for Ceruloplasmin antibody 21131-1-APDownload protocol
IHC protocol for Ceruloplasmin antibody 21131-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Cells

Phaseolus vulgaris Erythroagglutinin (PHA-E)-Positive Ceruloplasmin Acts as a Potential Biomarker in Pancreatic Cancer Diagnosis

Authors - Shanshan Sha
humanWB

J Clin Pharmacol

Expression of ceruloplasmin in the peripheral blood of patients with drug-resistant epilepsy

Authors - Hai-Yan Chen
mouseWB,IF

Cell Death Dis

The divergent effects of astrocyte ceruloplasmin on learning and memory function in young and old mice

Authors - Zhong-Da Li
mouseWB

iScience

Hepatic depletion of nucleolar protein mDEF causes excessive mitochondrial copper accumulation associated with p53 and NRF1 activation

Authors - Jinsong Wei
mouseWB

Mol Neurobiol

Gemfibrozil Alleviates Cognitive Impairment by Inhibiting Ferroptosis of Astrocytes via Restoring the Iron Metabolism and Promoting Antioxidant Capacity in Type 2 Diabetes

Authors - Nan Wang