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Ceruloplasmin Polyclonal antibody

Ceruloplasmin Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21131-1-AP

Synonyms

Ceruloplasmin, ceruloplasmin (ferroxidase), CP, CP 2, Ferroxidase

Validation Data Gallery

  • WB analysis of human plasma using 21131-1-AP

    WB analysis of human plasma using 21131-1-AP

    human blood were subjected to SDS PAGE followed by western blot with 21131-1-AP (Ceruloplasmin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human hepatocirrhosis using 21131-1-AP

    IHC staining of human hepatocirrhosis using 21131-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 21131-1-AP (Ceruloplasmin antibody) at dilution of 1:200 (under 10x lens).

Tested Applications

Positive WB detected inhuman blood tissue, mouse brain tissue, human plasma tissue
Positive IHC detected inhuman hepatocirrhosis tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21131-1-AP is Ceruloplasmin Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Ceruloplasmin fusion protein Ag15535
Full Name ceruloplasmin (ferroxidase)
Calculated molecular weight 1065 aa, 122 kDa
Observed molecular weight 130-140 kDa, 85 kDa
GenBank accession numberBC146663
Gene symbol CP
Gene ID (NCBI) 1356
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ceruloplasmin (CP) is a serum glycoprotein synthesized by hepatocytes and functions as the major transport protein of copper in blood where it acts as ferrodoxidase and is required for iron transport of transferrin. The intact ceruloplasmin migrated around 130-140 kDa and some proteolytic cleaved fragments could also be observed.

Protocols

Product Specific Protocols
WB protocol for Ceruloplasmin antibody 21131-1-APDownload protocol
IHC protocol for Ceruloplasmin antibody 21131-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols