CPA4 Polyclonal antibody

CPA4 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 26824-1-AP

Synonyms

Carboxypeptidase A3, carboxypeptidase A4, CPA3, CPA4



Tested Applications

Positive WB detected inmouse heart tissue, SMMC-7721 cells, mouse kidney tissue, A549 cells, HepG2 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 26824-1-AP is CPA4 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CPA4 fusion protein Ag25327
Full Name carboxypeptidase A4
Calculated molecular weight 421 aa, 47 kDa
Observed molecular weight 44 kDa, 150 kDa
GenBank accession numberBC052289
Gene symbol CPA4
Gene ID (NCBI) 51200
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CPA4 antibody 26824-1-APDownload protocol
IHC protocol for CPA4 antibody 26824-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Sci Signal

Hypoxic cancer-associated fibroblasts increase NCBP2-AS2/HIAR to promote endothelial sprouting through enhanced VEGF signaling.

Authors - Fernanda G Kugeratski