|Positive WB detected in||Y79 cells, rat testis tissue|
|Positive IHC detected in||mouse brain tissue, human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||Hela cells, MCF-7 cells|
|Western Blot (WB)||WB : 1:300-1:800|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
13474-1-AP targets Cryptochrome 1 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Cryptochrome 1 fusion protein Ag4270|
|Full Name||cryptochrome 1 (photolyase-like)|
|Calculated molecular weight||586 aa, 66 kDa|
|Observed molecular weight||70 kDa|
|GenBank accession number||BC030519|
|Gene ID (NCBI)||1407|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Cryptochrome circadian clock 1 (CRY1) is a flavin adenine dinucleotide-binding protein with MW of 66 kDa. CRY1 is a key component of the circadian core oscillator complex, which regulates the circadian clock. CRY1 predominantly displays evening-time expression and serves as a strong repressor of morning-time elements (E box/E-prime box) when bound to the BMAL1 (ARNTL; 602550)/CLOCK (601851) complex (PubMed: 21236481). This antibody can recognize CRY1 and CRY2 due to the high homology.
BMC Mol Cell Biol
Bromide impairs the circadian clock and glycolytic homeostasis via disruption of autophagy in rat H9C2 cardiomyocytes.
Cell Death Dis
hPER3 promotes adipogenesis via hHSP90AA1-mediated inhibition of Notch1 pathway.
Free Radic Biol Med
Hydrogen peroxide modulates clock gene expression via PRX2-STAT3-REV-ERBα/β pathway.
Stem Cell Reports
SCA1(+) Cells from the Heart Possess a Molecular Circadian Clock and Display Circadian Oscillations in Cellular Functions.
Circadian networks in human embryonic stem cell-derived cardiomyocytes.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Iru (Verified Customer) (06-26-2020)
It works fine in 1:1000.