Cryptochrome 1 Polyclonal antibody

Cryptochrome 1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13474-1-AP

Synonyms

CRY1, Cryptochrome 1, PHLL1



Tested Applications

Positive WB detected inY79 cells, rat testis tissue
Positive IHC detected inmouse brain tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:300-1:800
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 4 publications below

Product Information

13474-1-AP targets Cryptochrome 1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cryptochrome 1 fusion protein Ag4270
Full Name cryptochrome 1 (photolyase-like)
Calculated molecular weight 586 aa, 66 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC030519
Gene symbol CRY1
Gene ID (NCBI) 1407
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cryptochrome circadian clock 1 (CRY1) is a flavin adenine dinucleotide-binding protein with MW of 66 kDa. CRY1 is a key component of the circadian core oscillator complex, which regulates the circadian clock. CRY1 predominantly displays evening-time expression and serves as a strong repressor of morning-time elements (E box/E-prime box) when bound to the BMAL1 (ARNTL; 602550)/CLOCK (601851) complex (PubMed: 21236481). This antibody can recognize CRY1 and CRY2 due to the high homology.

Protocols

Product Specific Protocols
WB protocol for Cryptochrome 1 antibody 13474-1-APDownload protocol
IHC protocol for Cryptochrome 1 antibody 13474-1-APDownload protocol
IF protocol for Cryptochrome 1 antibody 13474-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

BMC Mol Cell Biol

Bromide impairs the circadian clock and glycolytic homeostasis via disruption of autophagy in rat H9C2 cardiomyocytes.

Authors - Yicheng Jiang
mouseWB

Free Radic Biol Med

Hydrogen peroxide modulates clock gene expression via PRX2-STAT3-REV-ERBα/β pathway.

Authors - Guohua Ji
humanWB

Stem Cell Reports

SCA1(+) Cells from the Heart Possess a Molecular Circadian Clock and Display Circadian Oscillations in Cellular Functions.

Authors - Bastiaan C Du Pré
humanWB

EMBO Rep

Circadian networks in human embryonic stem cell-derived cardiomyocytes.

Authors - Pieterjan Dierickx

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Iru (Verified Customer) (06-26-2020)

It works fine in 1:1000.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Fallopian tube epithelial cells