- Featured Product
- KD/KO Validated
CYP1A1 Polyclonal antibody
CYP1A1 Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IP, IHC, IF, FC,ELISA
Conjugate
Unconjugated
Cat no : 13241-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse cerebellum tissue, mouse lung tissue, HepG2 cells, HeLa cells, K-562 cells |
| Positive IP detected in | mouse lung tissue |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
| Positive FC detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 18 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
13241-1-AP targets CYP1A1 in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CYP1A1 fusion protein Ag3990 |
| Full Name | cytochrome P450, family 1, subfamily A, polypeptide 1 |
| Calculated molecular weight | 512 aa, 58 kDa |
| Observed molecular weight | 50-58 kDa |
| GenBank accession number | BC023019 |
| Gene symbol | CYP1A1 |
| Gene ID (NCBI) | 1543 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CYP1A1 antibody 13241-1-AP | Download protocol |
| IHC protocol for CYP1A1 antibody 13241-1-AP | Download protocol |
| IF protocol for CYP1A1 antibody 13241-1-AP | Download protocol |
| IP protocol for CYP1A1 antibody 13241-1-AP | Download protocol |
| FC protocol for CYP1A1 antibody 13241-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
ACS Omega Khellinoflavanone, a Semisynthetic Derivative of Khellin, Overcomes Benzo[a]pyrene Toxicity in Human Normal and Cancer Cells That Express CYP1A1. | ||
Cell Biol Int The AhR is involved in the regulation of LoVo cell proliferation through cell cycle-associated proteins. | ||
FEBS Open Bio Knockout of cytochrome P450 1A1 enhances lipopolysaccharide-induced acute lung injury in mice by targeting NF-κB activation.
| ||
Int J Mol Med Particulate matter 2.5 regulates lipid synthesis and inflammatory cytokine production in human SZ95 sebocytes. |
