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- KD/KO Validated
CYP1A1 Polyclonal antibody
CYP1A1 Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, pig and More (2)
Applications
WB,IP,IHC,IF,FC,ELISA
Conjugate
Unconjugated
Cat no : 13241-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse cerebellum tissue, mouse lung tissue, HepG2 cells, HeLa cells, K-562 cells |
| Positive IP detected in | mouse lung tissue |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
| Positive FC detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| Flow Cytometry (FC) | FC : 0.50 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 59 publications below |
| IHC | See 9 publications below |
| IF | See 9 publications below |
Product Information
13241-1-AP targets CYP1A1 in WB,IP,IHC,IF,FC,ELISA applications and shows reactivity with human, mouse, pig samples.
| Tested Reactivity | human, mouse, pig |
| Cited Reactivity | human, mouse, rat, zebrafish, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CYP1A1 fusion protein Ag3990 |
| Full Name | cytochrome P450, family 1, subfamily A, polypeptide 1 |
| Calculated Molecular Weight | 512 aa, 58 kDa |
| Observed Molecular Weight | 50-58 kDa |
| GenBank Accession Number | BC023019 |
| Gene Symbol | CYP1A1 |
| Gene ID (NCBI) | 1543 |
| RRID | AB_2877928 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CYP1A1 antibody 13241-1-AP | Download protocol |
| IHC protocol for CYP1A1 antibody 13241-1-AP | Download protocol |
| IF protocol for CYP1A1 antibody 13241-1-AP | Download protocol |
| IP protocol for CYP1A1 antibody 13241-1-AP | Download protocol |
| FC protocol for CYP1A1 antibody 13241-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Environ Int Novel fluorescent and secreted transcriptional reporters for quantifying activity of the xenobiotic sensor aryl hydrocarbon receptor (AHR) | ||
Free Radic Biol Med 5-Methoxyflavone ameliorates non-alcoholic fatty liver disease through targeting the cytochrome P450 1A1 | ||
Phytother Res Quercetin ameliorates ulcerative colitis by activating aryl hydrocarbon receptor to improve intestinal barrier integrity | ||
Environ Pollut Regioselective hydroxylation of carbendazim by mammalian cytochrome P450: A combined experimental and computational study. | ||
BMC Med NUPR1 contributes to radiation resistance by maintaining ROS homeostasis via AhR/CYP signal axis in hepatocellular carcinoma | ||
J Gastroenterol Ferroptosis in the colon epithelial cells as a therapeutic target for ulcerative colitis |
