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DUSP22 Polyclonal antibody

DUSP22 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 51005-2-AP

Synonyms

DUSP22, JKAP, JNK stimulatory phosphatase 1, JSP 1, JSP1, LMW DSP2, LMWDSP2, MAP kinase phosphatase x, MKP x, MKPX, VHX

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using DUSP22 Polyclonal antibody (51005-2-AP)

    WB analysis of HEK-293 using 51005-2-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 51005-2-AP (DUSP22 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using DUSP22 Polyclonal antibody (51005-2-AP)

    IHC staining of human lung cancer using 51005-2-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 51005-2-AP (DUSP22 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using DUSP22 Polyclonal antibody (51005-2-AP)

    IHC staining of human lung cancer using 51005-2-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 51005-2-AP (DUSP22 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using DUSP22 Polyclonal antibody (51005-2-AP)

    IHC staining of human lung cancer using 51005-2-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 51005-2-AP (DUSP22 antibody) at dilution of 1:10 (under 10x lens).

Tested Applications

Positive WB detected inHEK-293 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

51005-2-AP targets DUSP22 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name dual specificity phosphatase 22
Calculated Molecular Weight 184 aa, 21 kDa
Observed Molecular Weight 21 kDa
GenBank Accession NumberBC022847
Gene Symbol DUSP22
Gene ID (NCBI) 56940
RRIDAB_2230783
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for DUSP22 antibody 51005-2-APDownload protocol
IHC protocol for DUSP22 antibody 51005-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols