DUSP7/PYST2 Polyclonal antibody

DUSP7/PYST2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26910-1-AP

Synonyms

dual specificity phosphatase 7, DUSP7, MKP X, MKPX, PYST2



Tested Applications

Positive WB detected inrat heart tissue, mouse heart tissue, mouse kidney tissue
Positive IHC detected inhuman breast cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 3 publications below

Product Information

The immunogen of 26910-1-AP is DUSP7/PYST2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DUSP7/PYST2 fusion protein Ag25488
Full Name dual specificity phosphatase 7
Observed molecular weight 45-50 kDa
GenBank accession numberBC104880
Gene symbol DUSP7
Gene ID (NCBI) 1849
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Human dual-specificity phosphatase 7 (DUSP7/PYST2) is a member of a structurally homologous subfamily of MAP kinase phosphatases. DUSP7 is overexpressed in myeloid leukemia and other malignancies. It is known to bind and dephosphorylate ErkII, and as it, along with the other members of the DUSP family expresses high selectively for MAP kinases. It has been suggested that it functions as a method for selectively activating/deactivating different members of that family.

Protocols

Product Specific Protocols
WB protocol for DUSP7/PYST2 antibody 26910-1-APDownload protocol
IHC protocol for DUSP7/PYST2 antibody 26910-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Addict Biol

Dual-specificity phosphatase 15 (DUSP15) in the nucleus accumbens is a novel negative regulator of morphine-associated contextual memory.

Authors - Xiaomeng Qiao
humanWB

Br J Pharmacol

L-Hcy-induced cathepsin V mediates the vascular endothelial inflammation in hyperhomocysteinemia.

Authors - Yi-Ping Leng
humanWB

Mol Cancer

Linc-RoR promotes MAPK/ERK signaling and confers estrogen-independent growth of breast cancer.

Authors - Wan-Xin Peng