EMG1 Polyclonal antibody

EMG1 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 11965-1-AP

Synonyms

C2F, EMG1, Grcc2f, NEP1, Nucleolar protein EMG1 homolog, Protein C2f



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, PC-3 cells
Positive IP detected inHeLa cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

IFSee 1 publications below

Product Information

11965-1-AP targets EMG1 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EMG1 fusion protein Ag2575
Full Name EMG1 nucleolar protein homolog (S. cerevisiae)
Calculated molecular weight 244 aa, 27 kDa
Observed molecular weight 27 kDa
GenBank accession numberBC055314
Gene symbol EMG1
Gene ID (NCBI) 10436
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EMG1, also named as NEP1, is a highly conserved protein initially identified as “Essential for Mitotic Growth” in yeast. EMG1 is required for maturation of the 18S rRNA and biogenesis of the 40S ribosomal subunit. EMG1 is the methyltransferase in the biosynthesis of m1acp3-Psi in eukaryotic 18S rRNAs. It has an essential role in 40S ribosomal subunit biogenesis independent on its methyltransferase activity. A mutation in human EMG1 gene causes Bowen-Conradi syndrome (BCS), an autosomal recessive disorder characterized by severely impaired prenatal and postnatal growth, profound psychomotor retardation, and death in early childhood.

Protocols

Product Specific Protocols
WB protocol for EMG1 antibody 11965-1-APDownload protocol
IF protocol for EMG1 antibody 11965-1-APDownload protocol
IP protocol for EMG1 antibody 11965-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Biochim Biophys Acta

Growth arrest in the ribosomopathy, Bowen-Conradi syndrome, is due to dramatically reduced cell proliferation and a defect in mitotic progression.

Authors - Joy Armistead

Mol Cell Proteomics

Nucleolar enrichment of brain proteins with critical roles in human neurodevelopment.

Authors - Lukasz Slomnicki