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  • Featured Product
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EMG1 Polyclonal antibody

EMG1 Polyclonal Antibody for WB, IP, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 11965-1-AP

Synonyms

C2F, EMG1, Grcc2f, NEP1, Nucleolar protein EMG1 homolog, Protein C2f

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using EMG1 Polyclonal antibody (11965-1-AP)

    WB analysis of HEK-293 using 11965-1-AP

    WB result of EMG1 antibody (11965-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EMG1 transfected HEK-293 cells.

  • Western Blot (WB) analysis of HEK-293 cells using EMG1 Polyclonal antibody (11965-1-AP)

    WB analysis of HEK-293 using 11965-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11965-1-AP (EMG1 Antibody) at dilution of 1:3000 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of HeLa cells using EMG1 Polyclonal antibody (11965-1-AP)

    WB analysis of HeLa using 11965-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11965-1-AP (EMG1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using EMG1 Polyclonal antibody (11965-1-AP)

    WB analysis of PC-3 using 11965-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 11965-1-AP (EMG1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using EMG1 Polyclonal antibody (11965-1-AP)

    WB analysis of HeLa using 11965-1-AP

    WB result of EMG1 antibody (11965-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EMG1 transfected HeLa cells.

  • Immunoprecipitation (IP) experiment of HeLa cells using EMG1 Polyclonal antibody (11965-1-AP)

    IP experiment of HeLa using 11965-1-AP

    IP result of anti-EMG1 (IP:11965-1-AP, 4ug; Detection:11965-1-AP 1:400) with HeLa cells lysate 1080ug.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using EMG1 Polyclonal antibody (11965-1-AP)

    IF Staining of HeLa using 11965-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EMG1 antibody (11965-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, PC-3 cells
Positive IP detected inHeLa cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11965-1-AP targets EMG1 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse, goat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EMG1 fusion protein Ag2575
Full Name EMG1 nucleolar protein homolog (S. cerevisiae)
Calculated Molecular Weight 244 aa, 27 kDa
Observed Molecular Weight 27 kDa
GenBank Accession NumberBC055314
Gene Symbol EMG1
Gene ID (NCBI) 10436
RRIDAB_874093
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EMG1, also named as NEP1, is a highly conserved protein initially identified as "Essential for Mitotic Growth" in yeast. EMG1 is required for maturation of the 18S rRNA and biogenesis of the 40S ribosomal subunit. EMG1 is the methyltransferase in the biosynthesis of m1acp3-Psi in eukaryotic 18S rRNAs. It has an essential role in 40S ribosomal subunit biogenesis independent on its methyltransferase activity. A mutation in human EMG1 gene causes Bowen-Conradi syndrome (BCS), an autosomal recessive disorder characterized by severely impaired prenatal and postnatal growth, profound psychomotor retardation, and death in early childhood.

Protocols

Product Specific Protocols
WB protocol for EMG1 antibody 11965-1-APDownload protocol
IF protocol for EMG1 antibody 11965-1-APDownload protocol
IP protocol for EMG1 antibody 11965-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Mol Cell Proteomics

Nucleolar enrichment of brain proteins with critical roles in human neurodevelopment.

Authors - Lukasz Slomnicki
View Article
mouseIF

Biochim Biophys Acta

Growth arrest in the ribosomopathy, Bowen-Conradi syndrome, is due to dramatically reduced cell proliferation and a defect in mitotic progression.

Authors - Joy Armistead
View Article
goatWB

Int J Mol Sci

Integrative Proteomics and Transcriptomics Profiles of the Oviduct Reveal the Prolificacy-Related Candidate Biomarkers of Goats (Capra hircus) in Estrous Periods

Authors - Zhipeng Sun
View Article