FECH Polyclonal antibody

FECH Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 14466-1-AP

Synonyms

EPP, FCE, FECH, Ferrochelatase, mitochondrial, Heme synthase, Protoheme ferro lyase



Tested Applications

Positive WB detected inK-562 cells, human liver tissue, HepG2 cells, human heart tissue, mouse heart tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 8 publications below

Product Information

14466-1-AP targets FECH in WB, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FECH fusion protein Ag5814
Full Name ferrochelatase (protoporphyria)
Calculated molecular weight 48 kDa
Observed molecular weight 40-48 kDa
GenBank accession numberBC039841
Gene symbol FECH
Gene ID (NCBI) 2235
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FECH(ferrochelatase, mitochondrial ) is also named as heme synthase, protoheme ferro-lyase and belongs to the ferrochelatase family. It is a homodimeric (86 kDa) mitochondrial membrane-associated enzyme that catalyzes the insertion of ferrous iron into protoporphyrin to form heme(PMID:11175906). It has 2 isoforms produced by alternative splicing and the full length protein has a transit peptide with 54 amino acids.

Protocols

Product Specific Protocols
WB protocol for FECH antibody 14466-1-APDownload protocol
IHC protocol for FECH antibody 14466-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Haematologica

Dimeric ferrochelatase bridges ABCB7 and ABCB10 homodimers in an architecturally defined molecular complex required for heme biosynthesis.

Authors - Nunziata Maio
mouseWB

Cell Death Dis

Oxidized-LDL inhibits testosterone biosynthesis by affecting mitochondrial function and the p38 MAPK/COX-2 signaling pathway in Leydig cells.

Authors - Jun Jing
humanWB

Xenobiotica

Involvement of protoporphyrin IX accumulation in the pathogenesis of isoniazid/rifampicin-induced liver injury: the prevention of curcumin.

Authors - Leiyan He
ratWB

J Am Heart Assoc

MicroRNA-210 decreases heme levels by targeting ferrochelatase in cardiomyocytes.

Authors - Qiao Aijun A
mouseWB

Nat Commun

PMID: 32019928

Authors - Erika M Palmieri
humanWB

J Am Coll Cardiol

Heme levels are increased in human failing hearts.

Authors - Khechaduri Arineh A