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  • KD/KO Validated

NCS1 Polyclonal antibody

NCS1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10506-2-AP

Synonyms

FLUP, FREQ, Frequenin homolog, frequenin homolog (Drosophila), Frequenin like protein, NCS 1, NCS1, Neuronal calcium sensor 1



Tested Applications

Positive WB detected inmouse brain tissue, A431 cells, HepG2 cells, human brain tissue, mouse cerebellum tissue, PC-3 cells, rat brain tissue, SH-SY5Y cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman cerebellum tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10506-2-AP targets NCS1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NCS1 fusion protein Ag0784
Full Name frequenin homolog (Drosophila)
Calculated molecular weight 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC004856
Gene symbol FREQ
Gene ID (NCBI) 23413
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NCS1 is a member of the neuronal calcium sensor gene family. It regulates G protein-coupled receptor phosphorylation in a calcium-dependent manner and can substitute for calmodulin. The protein is associated with secretory granules and modulates synaptic transmission and synaptic plasticity.

Protocols

Product Specific Protocols
WB protocol for NCS1 antibody 10506-2-APDownload protocol
IHC protocol for NCS1 antibody 10506-2-APDownload protocol
IP protocol for NCS1 antibody 10506-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Sci Rep

Self-directed exploration provides a Ncs1-dependent learning bonus.

Authors - Ho-Suk Mun
  • KD Validated
mouseWB

Neuron

NCS-1 in the dentate gyrus promotes exploration, synaptic plasticity, and rapid acquisition of spatial memory.

Authors - Bechara J Saab