Filter: AllWBIPIHCIF-PFC (Intra)

WB analysis of mouse brain using 10506-2-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 10506-2-AP (NCS1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 10506-2-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 10506-2-AP (NCS1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 10506-2-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 10506-2-AP (NCS1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 10506-2-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 10506-2-AP (NCS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of SH-SY5Y using 10506-2-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10506-2-AP (NCS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 10506-2-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 10506-2-AP (NCS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 10506-2-AP
A431 cells were subjected to SDS PAGE followed by western blot with 10506-2-AP (NCS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse cerebellum using 10506-2-AP
mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 10506-2-AP (NCS1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 10506-2-AP
IP result of anti-NCS1 (IP:10506-2-AP, 4ug; Detection:10506-2-AP 1:1000) with mouse brain tissue lysate 4000ug.
IHC staining of human cerebellum using 10506-2-AP
Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 10506-2-AP (NCS1 antibody at dilution of 1:200 (under 10x lens).
IHC staining of human cerebellum using 10506-2-AP
Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 10506-2-AP (NCS1 antibody at dilution of 1:200 (under 40x lens).
IHC staining of human brain using 10506-2-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10506-2-AP (NCS1 antibody at dilution of 1:200 (under 10x lens).
IHC staining of human brain using 10506-2-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10506-2-AP (NCS1 antibody at dilution of 1:200 (under 40x lens).
IF Staining of mouse cerebellum using 10506-2-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using NCS1 antibody (10506-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse cerebellum using 10506-2-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using NCS1 antibody (10506-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse cerebellum using 10506-2-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using NCS1 antibody (10506-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
FC experiment of SH-SY5Y using 10506-2-AP
1X10^6 SH-SY5Y cells were intracellularly stained with 0.2 ug Anti-Human NCS1 (10506-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Featured Product
KD/KO Validated

NCS1 Polyclonal antibody

Name: NCS1 Polyclonal antibody
Brand: Proteintech
SKU: 10506-2-AP

NCS1 Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), IP, ELISA

Cat No. 10506-2-AP

Publications(5)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, FC (Intra), IP, CoIP, ELISA

Synonyms

FREQ, FLUP, Frequenin-like protein, Frequenin like protein, frequenin homolog (Drosophila)

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (5)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	mouse brain tissue, A431 cells, HepG2 cells, human brain tissue, mouse cerebellum tissue, PC-3 cells, rat brain tissue, SH-SY5Y cells
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human cerebellum tissue, human brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse cerebellum tissue
Positive FC (Intra) detected in	SH-SY5Y cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 5 publications below
CoIP	See 1 publications below

Product Information

10506-2-AP targets NCS1 in WB, IHC, IF-P, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag0784 Product name: Recombinant human FREQ protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-190 aa of BC004856 Sequence: MGKSNSKLKPEVVEELTRKTYFTEKEVQQWYKGFIKDCPSGQLDAAGFQKIYKQFFPFGDPTKFATFVFNVFDENKDGRIEFSEFIQALSVTSRGTLDEKLRWAFKLYDLDNDGYITRNEMLDIVDAIYQMVGNTVELPEEENTPEKRVDRIFAMMDKNADGKLTLQEFQEGSKADPSIVQALSLYDGLV Predict reactive species
Full Name	frequenin homolog (Drosophila)
Calculated Molecular Weight	22 kDa
Observed Molecular Weight	22 kDa
GenBank Accession Number	BC004856
Gene Symbol	NCS1
Gene ID (NCBI)	23413
RRID	AB_2105918
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P62166
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NCS1 is a member of the neuronal calcium sensor gene family. It regulates G protein-coupled receptor phosphorylation in a calcium-dependent manner and can substitute for calmodulin. The protein is associated with secretory granules and modulates synaptic transmission and synaptic plasticity.

Protocols

Product Specific Protocols
IF protocol for NCS1 antibody 10506-2-AP	Download protocol
IHC protocol for NCS1 antibody 10506-2-AP	Download protocol
IP protocol for NCS1 antibody 10506-2-AP	Download protocol
WB protocol for NCS1 antibody 10506-2-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Neuron NCS-1 in the dentate gyrus promotes exploration, synaptic plasticity, and rapid acquisition of spatial memory. Authors - Bechara J Saab View Article
mouse	WB	Sci Rep Self-directed exploration provides a Ncs1-dependent learning bonus. Authors - Ho-Suk Mun KD Validated View Article
mouse	WB,CoIP	Sci Transl Med Activation of the sigma-1 receptor chaperone alleviates symptoms of Wolfram syndrome in preclinical models Authors - Lucie Crouzier View Article
human	WB	Elife A hierarchical pathway for assembly of the distal appendages that organize primary cilia Authors - Tomoharu Kanie View Article
human	WB	Elife Myristoylated Neuronal Calcium Sensor-1 captures the preciliary vesicle at distal appendages Authors - Tomoharu Kanie KO Validated View Article