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  • KD/KO Validated

FUT4 Polyclonal antibody

FUT4 Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 19497-1-AP

Synonyms

Alpha (1,3) fucosyltransferase, ELFT, FCT3A, FUC TIV, Fucosyltransferase 4, Fucosyltransferase IV, FucT IV, FUT4, FUTIV



Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, HL-60 cells, HepG2 cells, SGC-7901 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman lung cancer tissue, human gliomas tissue, human skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inA375 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19497-1-AP targets FUT4 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific)
Calculated Molecular Weight 59 kDa
Observed Molecular Weight95-140 kDa
GenBank Accession NumberNM_002033
Gene Symbol FUT4
Gene ID (NCBI) 2526
RRIDAB_10733222
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FUT4, also named as ELFT and FCT3A, belongs to the glycosyltransferase 10 family. FUT4 may catalyze alpha-1,3 glycosidic linkages involved in the expression of Lewis X/SSEA-1 and VIM-2 antigens. The expression of CD15 (acts as a terminal glycotope in glycoproteins and glycolipids) is directed by FUT4 in promyelocytes and monocytes. FUT4 is an antigenic epitope defined as a Lewis X carbohydrate structure is expressed on murine embryonal carcinoma cells (EC), murine ES and iPS cells, and murine and human germ cells. It is widely used as a positive surface marker for mouse undifferentiated ES and iPS cells and a negative surface marker for human undifferentiated ES and iPS cells. Expression is down-regulated following differentiation of murine EC and ES cells, while the differentiation of human EC and ES cells is accompanied by an increase in FUT4 expression. FUT4 is associated with cell adhesion, migration and differentiation. 19497-1-AP antibody detects the glycosylated isoform proteins around 95-140 kDa in SDS-PAGE. (PMID: 28706275, 28914881, 11278338)

Protocols

Product Specific Protocols
WB protocol for FUT4 antibody 19497-1-APDownload protocol
IHC protocol for FUT4 antibody 19497-1-APDownload protocol
IP protocol for FUT4 antibody 19497-1-APDownload protocol
FC protocol for FUT4 antibody 19497-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

ACS Appl Mater Interfaces

Directing Induced Pluripotent Stem Cell Derived Neural Stem Cell Fate with a Three-Dimensional Biomimetic Hydrogel for Spinal Cord Injury Repair.

Authors - Lei Fan
humanWB

Cell Death Dis

α1,3-fucosylation of MEST promotes invasion potential of cytotrophoblast cells by activating translation initiation

Authors - Hao Wang
human,mouseWB,IF

Cell Death Differ

MicroRNA-200c impairs uterine receptivity formation by targeting FUT4 and α1,3-fucosylation.

Authors - Qin Zheng
  • KD Validated
humanWB,IF

Oncotarget

Ginsenoside Rg3 inhibits epithelial-mesenchymal transition (EMT) and invasion of lung cancer by down-regulating FUT4.

Authors - Lili Tian
  • KD Validated
humanWB

Cell Death Dis

Role of fucosyltransferase IV in epithelial-mesenchymal transition in breast cancer cells.

Authors - Yang X X
  • KD Validated
humanIF

Front Mol Neurosci

Transcriptional Repression of p53 by PAX3 Contributes to Gliomagenesis and Differentiation of Glioma Stem Cells.

Authors - Hui Zhu