Galectin-1 Polyclonal antibody

Galectin-1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11858-1-AP

Synonyms

14 kDa laminin binding protein, 14 kDa lectin, Gal 1, GAL1, Galaptin, Galectin 1, Galectin-1, HBL, HLBP14, HPL, Lactose binding lectin 1, LGALS1, S Lac lectin 1



Tested Applications

Positive WB detected inK-562 cells, human heart tissue, human liver tissue, L02 cells, NIH/3T3 cells, PC-12 cells
Positive IHC detected inhuman colon cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11858-1-AP targets Galectin-1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Galectin-1 fusion protein Ag2432
Full Name lectin, galactoside-binding, soluble, 1
Calculated molecular weight 15 kDa
Observed molecular weight 14 kDa
GenBank accession numberBC020675
Gene symbol LGALS1
Gene ID (NCBI) 3956
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-1 contains one carbohydrate recognition domain (CRD) and occurs as a monomer as well as a non-covalent homodimer (PMID: 16840800). It is differentially expressed by various normal and pathological tissues. Galectin-1 is a multifunctional protein that is involved in cell adhesion, migration, proliferation, apoptosis, inflammation, tumour transformation and growth (PMID:15785741).

Protocols

Product Specific Protocols
WB protocol for Galectin-1 antibody 11858-1-APDownload protocol
IHC protocol for Galectin-1 antibody 11858-1-APDownload protocol
IF protocol for Galectin-1 antibody 11858-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC,IF

J Exp Clin Cancer Res

Nuclear FAM289-Galectin-1 interaction controls FAM289-mediated tumor promotion in malignant glioma.

Authors - Xing Rong Guo
humanWB

BMC Microbiol

Global secretome characterization of A549 human alveolar epithelial carcinoma cells during Mycoplasma pneumoniae infection.

Authors - Shuxian Li
humanWB

Cell Rep

Hepatocellular Carcinomas Originate Predominantly from Hepatocytes and Benign Lesions from Hepatic Progenitor Cells.

Authors - Krishna S Tummala