Tested Applications
| Positive WB detected in | HT-1080 cells, HT1080 cells |
| Positive IP detected in | HT-1080 cells |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
Product Information
15189-1-AP targets GFPT2 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7275 Product name: Recombinant human GFPT2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 330-682 aa of BC000012 Sequence: IFEQPESVFNTMRGRVNFETNTVLLGGLKDHLKEIRRCRRLIVIGCGTSYHAAVATRQVLEELTELPVMVELASDFLDRNTPVFRDDVCFFISQSGETADTLLALRYCKDRGALTVGVTNTVGSSISRETDCGVHINAGPEVGVASTKAYTSQFISLVMFGLMMSEDRISLQNRRQEIIRGLRSLPELIKEVLSLEEKIHDLALELYTQRSLLVMGRGYNYATCLEGALKIKEITYMHSEGILAGELKHGPLALIDKQMPVIMVIMKDPCFAKCQNALQQVTARQGRPIILCSKDDTESSKFAYKTIELPHTVDCLQGILSVIPLQLLSFHLAVLRGYDVDFPRNLAKSVTVE Predict reactive species |
| Full Name | glutamine-fructose-6-phosphate transaminase 2 |
| Calculated Molecular Weight | 77 kDa |
| Observed Molecular Weight | 75-77 kDa |
| GenBank Accession Number | BC000012 |
| Gene Symbol | GFPT2 |
| Gene ID (NCBI) | 9945 |
| RRID | AB_2878116 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O94808 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GFPT2 (Glutamine-fructose-6phosphate transaminase 2) is a rate-limiting enzyme in hexosamine biosynthesis involved in the occurrence and progress of many cancers (PMID: 35330716, 14764791). GFPT2 plays an important role in the metabolic activity of cells, especially in their glucose metabolism. GFPT2 expression was highly correlated with EMT-related factors (PMID: 37395335). High GFPT2 expression is associated with poor prognosis in various tumors, including lung adenocarcinoma, colorectal cancer, leiomyosarcoma, and serous ovarian cancer.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for GFPT2 antibody 15189-1-AP | Download protocol |
| IP protocol for GFPT2 antibody 15189-1-AP | Download protocol |
| WB protocol for GFPT2 antibody 15189-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Res Mannose antagonizes GSDME-mediated pyroptosis through AMPK activated by metabolite GlcNAc-6P | ||
Sci China Life Sci Deamidation enables pathogenic SMAD6 variants to activate the BMP signaling pathway | ||
Prog Neuropsychopharmacol Biol Psychiatry Dihydroartemisinin promotes tau O-GlcNAcylation and improves cognitive function in hTau transgenic mice | ||
J Mol Cell Cardiol A roadmap for the characterization of energy metabolism in human cardiomyocytes derived from induced pluripotent stem cells |
