|Positive WB detected in||HeLa cells, mouse lung tissue, rat testis tissue, mouse testis tissue|
|Positive IP detected in||mouse testis tissue|
|Positive IHC detected in||human colon cancer tissue, human placenta tissue, human colon tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
19112-1-AP targets GOLPH3 in WB, IP, IHC, IF, CoIP,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||GOLPH3 fusion protein Ag5443|
|Full Name||golgi phosphoprotein 3 (coat-protein)|
|Calculated molecular weight||298 aa, 34 kDa|
|Observed molecular weight||34 kDa|
|GenBank accession number||BC033725|
|Gene ID (NCBI)||64083|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
GOLPH3 (also called GPP34, GMx33, MIDAS, or yeast Vps74p) is a 34-kDa Golgi-associated protein conserved from yeast to human. GOLPH3 binds to PtdIns(4)P-rich trans-Golgi membranes and MYO18A conveying a tensile force required for efficient tubule and vesicle formation (PMID: 19837035). GOLPH3 has been recently demonstrated as a novel oncoprotein amplified in various types of human malignancies, including melanoma, breast, non-small cell lung cancer, gliomas and connective tissue tumors (PMID:19553991; 23006319; 21499727; 22745132). Enhanced activation of mTOR signalling represents a molecular basis for the oncogenic activity of GOLPH3 (PMID: 19553991).
|Product Specific Protocols|
|WB protocol for GOLPH3 antibody 19112-1-AP||Download protocol|
|IHC protocol for GOLPH3 antibody 19112-1-AP||Download protocol|
|IF protocol for GOLPH3 antibody 19112-1-AP||Download protocol|
|IP protocol for GOLPH3 antibody 19112-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Re-evaluating the roles of myosin 18Aα and F-actin in determining Golgi morphology.
FAP-a and GOLPH3 Are Hallmarks of DCIS Progression to Invasive Breast Cancer.
GOLPH3 is a potential therapeutic target and a prognostic indicator of poor survival in bladder cancer treated by cystectomy.
APOL1 C-Terminal Variants May Trigger Kidney Disease through Interference with APOL3 Control of Actomyosin.
Increased expression of Golgi phosphoprotein-3 is associated with tumor aggressiveness and poor prognosis of prostate cancer.
Overexpression of GOLPH3 is associated with poor clinical outcome in gastric cancer.