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GOLPH3 Antibody

GOLPH3 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 19112-1-AP

Synonyms

Coat protein GPP34, Golgi phosphoprotein 3, GOLPH3, GOPP1, GPP34, MIDAS



Tested Applications

Positive WB detected inHeLa cells, mouse lung tissue, rat testis tissue, mouse testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman colon cancer tissue, human placenta tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

19112-1-AP targets GOLPH3 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GOLPH3 fusion protein Ag5443
Full Name golgi phosphoprotein 3 (coat-protein)
Calculated molecular weight 298aa,34 kDa
Observed molecular weight 34 kDa
GenBank accession number BC033725
Gene symbol GOLPH3
Gene ID (NCBI) 64083
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GOLPH3 (also called GPP34, GMx33, MIDAS, or yeast Vps74p) is a 34-kDa Golgi-associated protein conserved from yeast to human. GOLPH3 binds to PtdIns(4)P-rich trans-Golgi membranes and MYO18A conveying a tensile force required for efficient tubule and vesicle formation (PMID: 19837035). GOLPH3 has been recently demonstrated as a novel oncoprotein amplified in various types of human malignancies, including melanoma, breast, non-small cell lung cancer, gliomas and connective tissue tumors (PMID:19553991; 23006319; 21499727; 22745132). Enhanced activation of mTOR signalling represents a molecular basis for the oncogenic activity of GOLPH3 (PMID: 19553991).

Protocols

Product Specific Protocols
WB protocol for GOLPH3 antibody 19112-1-APDownload protocol
IHC protocol for GOLPH3 antibody 19112-1-APDownload protocol
IF protocol for GOLPH3 antibody 19112-1-APDownload protocol
IP protocol for GOLPH3 antibody 19112-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Cytoskeleton (Hoboken)

Re-evaluating the roles of myosin 18Aα and F-actin in determining Golgi morphology.

Authors - Kyle Bruun
humanIHC

Front Oncol

FAP-a and GOLPH3 Are Hallmarks of DCIS Progression to Invasive Breast Cancer.

Authors - Li-Na Yu
humanWB

Oncotarget

GOLPH3 is a potential therapeutic target and a prognostic indicator of poor survival in bladder cancer treated by cystectomy.

Authors - Qing Zhang
humanWB

Cell Rep

APOL1 C-Terminal Variants May Trigger Kidney Disease through Interference with APOL3 Control of Actomyosin.

Authors - Sophie Uzureau
humanIHC

Diagn Pathol

Increased expression of Golgi phosphoprotein-3 is associated with tumor aggressiveness and poor prognosis of prostate cancer.

Authors - Hua Xing X
humanIHC

Tumour Biol

Overexpression of GOLPH3 is associated with poor clinical outcome in gastric cancer.

Authors - Hu Ben-Shun BS