HLA class I (HLA-C) Polyclonal antibody

HLA class I (HLA-C) Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15777-1-AP

Synonyms

D6S204, HLA C, HLA Cw, HLA Cw12, HLA JY3, HLAC, HLA-C, HLC C, MHC class I antigen Cw*7, PSORS1



Tested Applications

Positive WB detected inJurkat cells, HL-60 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

15777-1-AP targets HLA class I (HLA-C) in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HLA class I (HLA-C) fusion protein Ag8461
Full Name major histocompatibility complex, class I, C
Calculated molecular weight 366 aa, 41 kDa
Observed molecular weight 44 kDa
GenBank accession numberBC007814
Gene symbol HLA-C
Gene ID (NCBI) 3107
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). This class I molecules are polymorphic membrane glycoproteins composed of a heavy (alpha) chain (44 kDa) which is encoded by a HLA class I gene (HLA-A, B or C), and β2-microglobulin light (beta) chain (12 kDa). They are involved in the presentation of foreign antigens to the immune system. (PMID: 667938; 3375250)

Protocols

Product Specific Protocols
WB protocol for HLA class I (HLA-C) antibody 15777-1-APDownload protocol
IHC protocol for HLA class I (HLA-C) antibody 15777-1-APDownload protocol
IF protocol for HLA class I (HLA-C) antibody 15777-1-APDownload protocol
IP protocol for HLA class I (HLA-C) antibody 15777-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols