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  • Featured Product
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HPRT1 Monoclonal antibody

HPRT1 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1C3D1

Cat no : 67518-1-Ig

Synonyms

HGPRT, HGPRTase, HPRT, HPRT1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HPRT1 Monoclonal antibody (67518-1-Ig)

    WB analysis using 67518-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67518-1-Ig (HPRT1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. HPRT1 deficient cell line Sp2/0 was served as a negative control.

  • Western Blot (WB) analysis of HEK-293 cells using HPRT1 Monoclonal antibody (67518-1-Ig)

    WB analysis of HEK-293 using 67518-1-Ig

    WB result of HPRT1 antibody (67518-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HPRT1 transfected HEK-293 cells.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using HPRT1 Monoclonal antibody (67518-1-Ig)

    IHC staining of mouse liver using 67518-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67518-1-Ig (HPRT1 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using HPRT1 Monoclonal antibody (67518-1-Ig)

    IHC staining of mouse liver using 67518-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67518-1-Ig (HPRT1 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using HPRT1 Monoclonal antibody (67518-1-Ig)

    IHC staining of human endometrial cancer using 67518-1-Ig

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 67518-1-Ig (HPRT1 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using HPRT1 Monoclonal antibody (67518-1-Ig)

    IHC staining of human endometrial cancer using 67518-1-Ig

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 67518-1-Ig (HPRT1 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using HPRT1 Monoclonal antibody (67518-1-Ig)

    IHC staining of human liver cancer using 67518-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67518-1-Ig (HPRT1 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using HPRT1 Monoclonal antibody (67518-1-Ig)

    IHC staining of human colon cancer using 67518-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67518-1-Ig (HPRT1 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using HPRT1 Monoclonal antibody (67518-1-Ig)

    IHC staining of human colon cancer using 67518-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67518-1-Ig (HPRT1 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HPRT1 Monoclonal antibody (67518-1-Ig)

    IF Staining of HeLa using 67518-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 67518-1-Ig (HPRT1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inmouse liver tissue, human liver cancer tissue, human colon cancer tissue, human endometrial cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:4000-1:16000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67518-1-Ig targets HPRT1 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HPRT1 fusion protein Ag7044
Full Name hypoxanthine phosphoribosyltransferase 1
Calculated Molecular Weight 25 kDa
Observed Molecular Weight 24-28 kDa
GenBank Accession NumberBC000578
Gene Symbol HPRT1
Gene ID (NCBI) 3251
RRIDAB_2882738
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HPRT1, also named as HGPRT, plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutation of HPRT1 is associated with Lesch-Nyhan syndrome (LNS) which is an X-linked inherited neurogenetic disorder of purine metabolism. It has been reported that HPRT1 also plays an important role in HPRT-related gout. 67518-1-Ig antibody detects the protein around 28 kDa in SDS-PAGE. And HPRT1 deficient cell line Sp2/0 was served as a negative control in PTG's results.(PMID:20544509, 28045594, 27379977)

Protocols

Product Specific Protocols
WB protocol for HPRT1 antibody 67518-1-IgDownload protocol
IHC protocol for HPRT1 antibody 67518-1-IgDownload protocol
IF protocol for HPRT1 antibody 67518-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols